Western Blot (WB) (Western blotAll lanes: HNRPD antibody at 2ug/mlLane 1: 293T whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39,37,33,31 kDaObserved band size: 38 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human small intestine using MBS7001816 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human prostate using MBS7001816 at dilution of 1:100)
Western Blot (WB) (Western blotAll lanes: HNRNPA1 antibody at 6ug/mlLane 1: K562 whole cell lysateLane 2: A549 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39,35,30 kDaObserved band size: 39 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded mouse heart using MBS7002731 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7002731 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (All lanes: Heterogeneous nuclear ribonucleoprotein D0 antibody at 4ug/mlLane 1:HeLa whole cell lysateLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,37,33,31 kDaObserved band size: 38 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil using MBS1493245 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human endometrial using MBS1493245 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1490512 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1490512 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS1490512 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: Heterogeneous nuclear ribonucleoprotein H antibody at 8ug/mlLane 1: HepG-2 whole cell lysateLane 2: MCF7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 49 kDaObserved band size: 49 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7002558 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human cervical cancer tissue using MBS7002558 at dilution 1:100)
Immunofluorescence (IF) (MBS9601436 staining K-562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9601436 at 1/100 staining human Breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of hnRNP G Antibody expression in Hela cells lysates.The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescence (IF) (MBS9601436 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9601436 at 1/100 staining Rat ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Western Blot (WB) (Western blotAll lanes: PRMT6 antibody at 0.4ug/mlLane 1: 293T whole cell lysateLane 2: MCF7 whole cell lysateLane 3: A431 whole cell lysateLane 4: K562 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 42,33 kDaObserved band size: 42 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7006152 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7006152 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western Blot analysis of various cells using hnRNP K Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB) (Western blotAll lanes: HNRNPM antibody at 2ug/ml+NIH3T3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 78,74 kDaObserved band size: 77 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1497284 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1497284 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: PRMT3 antibody at 7 ug/ml+ Mouse brain tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 60,53 kDaObserved band size: 60 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded MBS7044199 using human brain tissue at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7044199 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of MCF7 cells using MBS7044199 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in:HepG2 whole cell lysate,Mouse heart tissue,Mouse brain tissue,Mouse kidney tissueAll lanes: HNRNPL antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65,51 kDaObserved band size: 65 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1491998 at dilution of 1:100)
Western Blot (WB) (Western Blot analysis of LoVo and A375 cell, Mouse brain tissue, HepG2, A431 and Hela cell using HNRNPH1 Polyclonal Antibody at dilution of 1:100)
Western Blot (WB) (Western Blot analysis of various cells with hnRNP H Polyclonal Antibody.)
Western Blot (WB) (Western Blot analysis of K562 cell using HNRNPC Polyclonal Antibody at dilution of 1/200)
Western Blot (WB) (Western blot analysis of extracts ofvarious cell lines, using FUS antibody (MBS9127265) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB) (Western blot analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody (MBS9127265) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% non fat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using FUS antibody (MBS9127265) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using FUS antibody (MBS9127265) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using FUS antibody (MBS9127265) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse spleen using FUS antibody (MBS9127265) at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of C6 cells using FUS antibody (MBS9127265) at dilution of1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of NIH/3T3 cells using FUS antibody (MBS9127265) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western Blot analysis of K562 and 293T cell using HNRNPC Polyclonal Antibody at dilution of 1/200)
Activity Data (Cold-inducible RNA-binding protein (CIRBP) plays a critical role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. Besides, Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) has been identified as an interactor of CIRBP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse CIRBP and recombinant mouse HNRPA1. Briefly, CIRBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HNRPA1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CIRBP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and HNRPA1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Sequence
Gene Sequencing (extract) (Sample: Active recombinant CIRBP, Mouse)
SDS-PAGE (Sample: Active recombinant CIRBP, Mouse)
Western Blot (WB) (Sample: Recombinant CIRBP, Mouse; Antibody: Rabbit Anti-Mouse CIRBP Ab)
Western Blot (WB) (Western blot analysis of hnRNP A2/B1 expression in Mouse lung lysate)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using hnRNP A2/B1 antibody.)
Immunofluorescence (IF) (MBS9604585 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF) (MBS9604666 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using hnRNP F antibody.)
Immunofluorescence (IF) (MBS9600366 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9600366 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF) (MBS9600366 staining CACO-2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis on HT29 cell lysate using hnRNP M Antibody, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9607644 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of HeLa, using PCBP2antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9604538 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells using hnRNP C1/C2 antibody.)
Immunofluorescence (IF) (MBS9604538 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis of hnRNP C1/2 phosphorylation expression in H2O2 treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9600890 at 1/200 staining human colon carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of Phospho-hnRNP C1/2 (Ser260) expression in various lysates)
Immunofluorescence (IF) (MBS9600890 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF) (MBS9601593 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of hnRNP K expression in LOVO cells)
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using HNRNPH2 antibody (MBS9133045) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunofluorescence (IF) (Immunofluorescence analysis of 293T cells using HNRNPH2 antibody (MBS9133045) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysateAll lanes: RALY antibody at 5.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 33, 31 KDaObserved band size: 33 KDa)
Immunohistochemistry (IHC) (IHC image of MBS7107691 diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (IHC image of MBS7107691 diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF) (Immunofluorescence staining of Hela cells with MBS7107691 at 1:167, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC) (MBS9604542 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of hnRNP A1 expression in mouse brain lysate)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using hnRNP A1 antibody.)
Immunofluorescence (IF) (MBS9604542 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using hnRNP G antibody.)
Immunofluorescene (IF) (MBS9604633 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9601434 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of hnRNP C1/C2 Antibody expression in 293 cells lysates.The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescence (IF) (MBS9601434 staining K-562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF) (MBS9601434 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HNRNPU antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
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