Rabbit anti-Human Heterogeneous nuclear ribonucleoprotein A1 Polyclonal Antibody | anti-HNRNPA1 antibody

Rabbit anti-human Heterogeneous nuclear ribonucleoprotein A1 polyclonal Antibody

Cat. Num. AAA7002731

• Gene Names: HNRNPA1; UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Heterogeneous nuclear ribonucleoprotein A1; Polyclonal Antibody; Rabbit anti-human Heterogeneous nuclear ribonucleoprotein A1 polyclonal Antibody; Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1; HNRNPA1; HNRPA1; anti-HNRNPA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 320

• Applicable Applications for anti-HNRNPA1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC: 1:20-1:200
• Immunogen: Recombinant human Heterogeneous nuclear ribonucleoprotein A1 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10030 / sc-10029 / sc-10032
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: HNRNPA1 antibody at 6ug/mlLane 1: K562 whole cell lysateLane 2: A549 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39,35,30 kDaObserved band size: 39 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embeded mouse heart using MBS7002731 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7002731 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-HNRNPA1 antibody

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites.

Product Categories/Family for anti-HNRNPA1 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4504445
• NCBI GeneID: 3178
• NCBI Accession #: NP_002127.1
• NCBI GenBank Nucleotide #: NM_002136.3
• UniProt Accession #: P09651; Q3MIB7; Q6PJZ7; A8K4Z8
• Molecular Weight: 29,386 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein A1 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein A1
• NCBI Official Symbol: HNRNPA1
• NCBI Official Synonym Symbols: UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein A1
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein A1
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPA1
• UniProt Synonym Gene Names: HNRPA1; hnRNP A1
• UniProt Entry Name: ROA1_HUMAN

NCBI Description

This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016]

Uniprot Description

hnRNP A1: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear export; RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; ribonucleoprotein complex; spliceosome

Molecular Function: nucleotide binding; protein binding; RNA binding; single-stranded DNA binding; single-stranded RNA binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; gene expression; mRNA processing; mRNA transport; negative regulation of telomere maintenance via telomerase; nuclear export; nuclear import; nuclear mRNA splicing, via spliceosome; positive regulation of telomere maintenance via telomerase; RNA export from nucleus; RNA splicing; viral reproduction

Disease: Amyotrophic Lateral Sclerosis 20; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 3