Western Blot (WB)
(Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Rabbit anti-Human Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) Polyclonal Antibody | anti-HNRPA2B1 antibody
Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1)
MGHHHHHHSGSEF-RKLFIGGLSF ETTEESLRNY YEQWGKLTDC VVMRDPASKR SRGFGFVTFS SMAEVDAAMA ARPHSIDGRV VEPKRAVARE ESG
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HNRPA2B1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HNRPA2B1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: HNRPA2B1 knockout A549 cell lysate; Predicted MW: 37kd Observed MW: 37kd Primary Ab: 5ug/ml Rabbit Anti-Human HNRPA2B1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: HNRPA2B1 knockout A549 cell lysate; Predicted MW: 37kd Observed MW: 37kd Primary Ab: 5ug/ml Rabbit Anti-Human HNRPA2B1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP A2/B1: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA splicing; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 7p15.2
Cellular Component: Cajal body; catalytic step 2 spliceosome; chromosome, telomeric region; cytoplasm; extracellular exosome; intracellular ribonucleoprotein complex; membrane; nuclear matrix; nucleoplasm; nucleus; spliceosomal complex
Molecular Function: G-rich strand telomeric DNA binding; miRNA binding; mRNA 3'-UTR binding; N6-methyladenosine-containing RNA binding; protein binding; RNA binding; single-stranded telomeric DNA binding
Biological Process: G-quadruplex DNA unwinding; miRNA transport; mRNA export from nucleus; mRNA processing; mRNA splicing, via spliceosome; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of transcription from RNA polymerase II promoter; positive regulation of telomerase RNA reverse transcriptase activity; positive regulation of telomere maintenance via telomere lengthening; primary microRNA processing; RNA metabolic process; RNA transport
Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 2
Research Articles on HNRPA2B1
Similar Products
Product Notes
The HNRPA2B1 hnrnpa2b1 (Catalog #AAA2003709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HNRPA2B1 hnrnpa2b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-RKLFIG GLSF ETTEESLRNY YEQWGKLTDC VVMRDPASKR SRGFGFVTFS SMAEVDAAMA ARPHSIDGRV VEPKRAVARE ESG. It is sometimes possible for the material contained within the vial of "Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
