Hamster Hnrnp-K ELISA Kit | HNRNPAB elisa kit

Hamster Hnrnp-K ELISA Kit

Cat. Num. AAA15641

• Gene Names: HNRNPAB; ABBP1; HNRPAB
• Reactivity: Hamster
• Synonyms: Hnrnp-K; Hamster Hnrnp-K ELISA Kit; HNRNPAB elisa kit

• Reactivity: Hamster
• Specificity: This kit recognizes recombinant and natural Human HNRNPAB, no significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 0.25 ng/ml ~ 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human HNRNPAB in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human HNRNPAB concentration. The concentration of Human HNRNPAB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNRNPAB elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 158523286
• NCBI GeneID: 3182
• UniProt Accession #: Q99729; Q04150; Q8N7U3; Q9BQ99; B3KNN5; D3DWP7
• Molecular Weight: 36,225 Da
• NCBI Official Full Name: Heterogeneous nuclear ribonucleoprotein A/B
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein A/B
• NCBI Official Symbol: HNRNPAB
• NCBI Official Synonym Symbols: ABBP1; HNRPAB
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein A/B; ABBP-1; hnRNP A/B; hnRNP type A/B protein; APOBEC1-binding protein 1; apobec-1 binding protein 1; apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1-binding protein 1
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein A/B
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPAB
• UniProt Synonym Gene Names: ABBP1; HNRPAB; hnRNP A/B; ABBP-1
• UniProt Entry Name: ROAA_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP A/B: a single-stranded RNA binding protein with two RNA-recognition (RRM) domains. Has a high affinity for G- rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site. Interacts with APOBEC1. Observed in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. A member of the RNA recognition motif (RRM) family. Four isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: nucleoplasm; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; sequence-specific DNA binding; RNA binding; nucleotide binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter