Monkey Secretin ELISA Kit | SCT elisa kit

Monkey Secretin ELISA Kit

Cat. Num. AAA737722

• Reactivity: Monkey
• Synonyms: Secretin; Monkey Secretin ELISA Kit; SCT elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCT elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This SCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SCT. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY SCT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SCT. Standards or samples are then added to the microtiter plate wells and SCT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SCT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SCT are added to each well to “sandwich” the SCT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SCT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCT concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SCT elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 11138667
• NCBI GeneID: 6343
• Molecular Weight: 13,016 Da
• NCBI Official Full Name: secretin
• NCBI Official Synonym Full Names: secretin
• NCBI Official Symbol: SCT
• NCBI Protein Information: secretin; prepro-secretin
• UniProt Protein Name: Secretin
• Protein Family: Secretin
• UniProt Gene Name: SCT
• UniProt Entry Name: SECR_HUMAN

NCBI Description

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. [provided by RefSeq, Jul 2008]

Uniprot Description

SCT: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: dentate gyrus development; visual learning; negative regulation of neuron apoptosis; pancreatic juice secretion