Porcine Secretin ELISA Kit | SCT elisa kit

Porcine Secretin ELISA Kit

Cat. Num. AAA739038

• Reactivity: Porcine
• Synonyms: Secretin; Porcine Secretin ELISA Kit; SCT elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCT elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This SCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SCT. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY SCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SCT antibody and an SCT-HRP conjugate. The assay sample and buffer are incubated together with SCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCT concentration since SCT from samples and SCT-HRP conjugate compete for the anti-SCT antibody binding site. Since the number of sites is limited, as more sites are occupied by SCT from the sample, fewer sites are left to bind SCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCT concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SCT elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 11138667
• NCBI GeneID: 6343
• Molecular Weight: 13,016 Da
• NCBI Official Full Name: secretin
• NCBI Official Synonym Full Names: secretin
• NCBI Official Symbol: SCT
• NCBI Protein Information: secretin; prepro-secretin
• UniProt Protein Name: Secretin
• Protein Family: Secretin
• UniProt Gene Name: SCT
• UniProt Entry Name: SECR_HUMAN

NCBI Description

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. [provided by RefSeq, Jul 2008]

Uniprot Description

SCT: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: dentate gyrus development; visual learning; negative regulation of neuron apoptosis; pancreatic juice secretion