Human SCT ELISA Kit | SCT elisa kit

Human SCT (Secretin) ELISA Kit

Cat. Num. AAA2504509

• Reactivity: Human
• Synonyms: SCT; Human SCT (Secretin) ELISA Kit; SCT elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human SCT. No significant cross-reactivity or interference between Human SCT and analogues was observed.
• Sequence Length: 121

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Competitive
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SCT concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCT elisa kit

Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SCT. During the reaction, SCT in the sample or standard competes with a fixed amount of SCT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SCT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SCT in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11138667
• NCBI GeneID: 6343
• UniProt Accession #: P09683
• Molecular Weight: 13,016 Da
• NCBI Official Full Name: secretin
• NCBI Official Synonym Full Names: secretin
• NCBI Official Symbol: SCT
• NCBI Protein Information: secretin; prepro-secretin
• UniProt Protein Name: Secretin
• Protein Family: Secretin
• UniProt Gene Name: SCT
• UniProt Entry Name: SECR_HUMAN

NCBI Description

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. [provided by RefSeq, Jul 2008]

Uniprot Description

SCT: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: dentate gyrus development; visual learning; negative regulation of neuron apoptosis; pancreatic juice secretion