Salmon Secretin, SCT ELISA Kit | SCT elisa kit

Salmon Secretin, SCT ELISA Kit

Cat. Num. AAA2602627

• Reactivity: Salmon
• Synonyms: Secretin; SCT; Salmon Secretin; SCT ELISA Kit; SCT elisa kit

• Reactivity: Salmon
• Specificity: No cross-reaction with other factors.

• Samples: Salmon serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SCT concentration
• Assay Type: Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCT elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Salmon SCT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 11345450
• NCBI GeneID: 6343
• NCBI Accession #: NP_068739.1
• NCBI GenBank Nucleotide #: NM_021920.2
• UniProt Accession #: P09683
• Molecular Weight: 13,016 Da
• NCBI Official Full Name: secretin preproprotein
• NCBI Official Synonym Full Names: secretin
• NCBI Official Symbol: SCT
• NCBI Protein Information: secretin; prepro-secretin
• UniProt Protein Name: Secretin
• Protein Family: Secretin
• UniProt Gene Name: SCT
• UniProt Entry Name: SECR_HUMAN

NCBI Description

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. [provided by RefSeq, Jul 2008]

Uniprot Description

SCT: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: dentate gyrus development; visual learning; negative regulation of neuron apoptosis; pancreatic juice secretion