S100A13 recombinant protein

S100A13 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122613

• Purity: >90% as determined by SDS-PAGE
• Synonyms: S100A13; S100A13 Protein; Mouse; Recombinant (His Tag); Mouse S100A13 Protein (His Tag); S100 calcium binding protein A13; S100A13 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys98

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse S100A13 (P97352)(Met1-Lys98) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for S100A13 recombinant protein

Background: S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from theneural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A13, also known as S100 calciumbinding protein A13, is a member of the S-100 family. It contains two EF-hand domains. S100A13 binds two calcium ions per subunit and one copper ion. Binding of one copper ion does not interfere with calcium-binding. S100A13 is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. S100A13 plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway.

Product Categories/Family for S100A13 recombinant protein

Calcium-binding Proteins and Related Molecules

References

Mandinova A. et al., 2003, J Cell Sci. 116: 2687-96. Arnesano F. et al., 2005, Angew Chem Int Ed. 44: 6341-4. Viemann D. et al., 2005, Blood. 105: 2955-62. Nakatani Y. et al., 2005, Mediators Inflamm. 2005: 280-92. Bjoerk P. et al., 2009, PLoS Biol. 7: E97-E97.

NCBI and Uniprot Product Information

• NCBI GI #: 2493417
• NCBI GeneID: 20196
• UniProt Accession #: P97352
• Molecular Weight: 11,158 Da
• NCBI Official Full Name: Protein S100-A13
• NCBI Official Synonym Full Names: S100 calcium binding protein A13
• NCBI Official Symbol: S100a13
• NCBI Protein Information: protein S100-A13
• UniProt Protein Name: Protein S100-A13
• Protein Family: Protein
• UniProt Gene Name: S100a13

Uniprot Description

S100A13: Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 3 F1|3 39.24 cM

Cellular Component: cytoplasm; cytosol; extracellular space; nucleus; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; copper ion binding; fibroblast growth factor binding; protein homodimerization activity; RAGE receptor binding; zinc ion binding

Biological Process: cytokine secretion; interleukin-1 alpha secretion; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of cell shape