Testing Data
E-Cadherin ELISA Pair Set | CDH1 elisa pair set
E-Cadherin Matched ELISA Antibody Pair Set, Human
Synonyms
E-Cadherin; E-Cadherin Matched ELISA Antibody Pair Set; Human; Human E-Cadherin/CDH1/E-cad/CD324 ELISA Pair Set; cadherin 1; type 1; E-cadherin (epithelial); Arc-1 Matched ELISA Antibody Pair Set; CD324 Matched ELISA Antibody Pair Set; CDH1 Matched ELISA Antibody Pair Set; CDHE Matched ELISA Antibody Pair Set; E-cad Matched ELISA Antibody Pair Set; ECAD Matched ELISA Antibody Pair Set; LCAM Matched ELISA Antibody Pair Set; UVO Matched ELISA Antibody Pair Set; CDH1 elisa pair set
Specificity
Quantitative determination of Human E-Cadherin
Assay Type
Solid Phase Sandwich ELISA
Detection Range
25-1600pg/mL
Sensitivity
25pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of mouse anti-Human E-Cadherin/CDH1/E-cad/CD324 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human E-Cadherin/CDH1/E-cad/CD324 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in Dilution Buffer before use.3. Standard Each vial contains 115 ng of recombinant Human E-Cadherin/CDH1/E-cad/CD324. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 1600 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human E-Cadherin/CDH1/E-cad/CD324 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in Dilution Buffer before use.3. Standard Each vial contains 115 ng of recombinant Human E-Cadherin/CDH1/E-cad/CD324. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 1600 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CDH1 elisa pair set
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
This E-Cadherin Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human E-Cadherin. It contains Human E-Cadherin capture antibody, Human E-Cadherin detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human E-Cadherin protein.
This E-Cadherin Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human E-Cadherin. It contains Human E-Cadherin capture antibody, Human E-Cadherin detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human E-Cadherin protein.
Product Categories/Family for CDH1 elisa pair set
References
Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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Product Notes
The CDH1 (Catalog #AAA8118212) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "E-Cadherin, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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