Typical Testing Data/Standard Curve (for reference only)
Mouse R-Spondin-3 ELISA Kit | RSPO3 elisa kit
Mouse R-Spondin-3 PicoKine ELISA Kit
Gene Names
Rspo3; Thsd2; AW742308; Cristin1; 2810459H04Rik
Reactivity
Mouse
Synonyms
R-Spondin-3; Mouse R-Spondin-3 PicoKine ELISA Kit; CRISTIN1; hPWTSR; hRspo3; PWTSR; R-spondin-3; R-spondin 3 homolog; R-spondin-3 [Precursor]; THSD2; Q2TJ95; RSPO3 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse R-Spondin-3
Sequence Length
277
Assay Type
Quantitative Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RSPO3 elisa kit
Principle of the Assay: Mouse R-Spondin-3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A specific for R-Spondin-3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q22-H277) and test samples are added to the wells, a biotinylated detection specific for R-Spondin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse R-Spondin-3 amount of sample captured in plate.
Background: R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the Rspondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
Background: R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the Rspondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,454 Da
NCBI Official Full Name
R-spondin-3
NCBI Official Synonym Full Names
R-spondin 3
NCBI Official Symbol
Rspo3
NCBI Official Synonym Symbols
Thsd2; AW742308; Cristin1; 2810459H04Rik
NCBI Protein Information
R-spondin-3
UniProt Protein Name
R-spondin-3
Protein Family
UniProt Gene Name
Rspo3
UniProt Synonym Gene Names
Cristin-1
UniProt Entry Name
RSPO3_MOUSE
Uniprot Description
R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide
Cellular Component: extracellular region
Molecular Function: frizzled binding; heparin binding; receptor binding
Biological Process: angiogenesis; blood vessel remodeling; sprouting angiogenesis
Research Articles on RSPO3
Similar Products
Product Notes
The Mouse RSPO3 rspo3 (Catalog #AAA178563) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178563 ELISA Kit recognizes Mouse RSPO3. It is sometimes possible for the material contained within the vial of "R-Spondin-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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