Mouse R-Spondin 3 (RSPO3) RTU ELISA Kit | RSPO3 rtu elisa kit

Mouse R-Spondin 3 (RSPO3) ELISA Kit

Cat. Num. AAA4503817

• Gene Names: Rspo3; Thsd2; AW742308; Cristin1; 2810459H04Rik
• Reactivity: Mouse
• Synonyms: R-Spondin 3 (RSPO3); Mouse R-Spondin 3 (RSPO3) ELISA Kit; Roof plate-specific spondin-3; Nucleopondin; Cabriolet; Cysteine-rich and single thrombospondin domain-containing protein 1(Cristin-1); RSPO3 rtu elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of RNASE3. No significant cross-reactivity or interference between RNASE3 and analogues was observed.
• Sequence Length: 277

• Samples: Human Serum, Plasma, Cell Lysates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 28.43pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNASE3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNASE3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Stable for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RSPO3 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RNASE3 in human serum, plasma, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNASE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RNASE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNASE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RNASE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 269973856
• NCBI GeneID: 72780
• NCBI Accession #: NP_082627.3
• NCBI GenBank Nucleotide #: NM_028351.3
• UniProt Accession #: Q2TJ95; Q3SYI9; Q5R2V4; Q8BVW2; Q9CSB2; A6H6M4
• Molecular Weight: 31,454 Da
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: Rspo3
• NCBI Official Synonym Symbols: Thsd2; AW742308; Cristin1; 2810459H04Rik
• NCBI Protein Information: R-spondin-3
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: Rspo3
• UniProt Synonym Gene Names: Cristin-11 PublicationManual assertion based on opinion iniRef.1

Uniprot Description

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis (PubMed:16543246, PubMed:21693646, PubMed:26766444). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed:16543246, PubMed:21693646). Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (PubMed:26766444).