Mouse anti-Human KPNA1 / Importin Alpha 5 Monoclonal Antibody | anti-KPNA1 antibody

Mouse Monoclonal [clone 2A4-1B5] (IgG1,k) to Human KPNA1 / Importin Alpha 5

Cat. Num. AAA246486

• Gene Names: KPNA1; RCH2; SRP1; IPOA5; NPI-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: KPNA1 / Importin Alpha 5; Monoclonal Antibody; Mouse Monoclonal [clone 2A4-1B5] (IgG1; k) to Human KPNA1 / Importin Alpha 5; Anti-KPNA1 / Importin Alpha 5 Antibody (clone 2A4-1B5) IHC-plus; KPNA1; Importin-alpha-S1; Karyopherin subunit alpha-1; NPI-1; Importin alpha 5; Importin subunit alpha-1; SRP1; SRP1-beta; RAG cohort protein 2; Importin alpha-1 subunit; IPOA5; Nucleoprotein interactor 1; RCH2; Human KPNA1; Importin Alpha 5; anti-KPNA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A4-1B5
• Specificity: Human Importin Alpha-1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 538

• Applicable Applications for anti-KPNA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: KPNA1 (AAH02374, 1 a.a. ~ 539 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: KPNA1 / Importin Alpha 5 antibody was raised against kPNA1 (AAH02374, 1 a.a. ~ 539 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-KPNA1 / SRP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of KPNA1 expression in transfected 293T cell line by KPNA1 monoclonal antibody.)

Western Blot (WB)

(Western blot of KPNA1 expression in HeLa cell lysate.)

ELISA (EIA)

(Detection limit for recombinant GST tagged KPNA1 is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-KPNA1 antibody

Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The KPNA1 product interacts with RAG1 and may play a role in V(D)J recombination.

NCBI and Uniprot Product Information

• NCBI GI #: 222144293
• NCBI GeneID: 3836
• NCBI Accession #: NP_002255.3
• NCBI GenBank Nucleotide #: NM_002264.3
• UniProt Accession #: P52294; Q6IBQ9; Q9BQ56; D3DN93
• Molecular Weight: 60,222 Da
• NCBI Official Full Name: importin subunit alpha-5
• NCBI Official Synonym Full Names: karyopherin alpha 1 (importin alpha 5)
• NCBI Official Symbol: KPNA1
• NCBI Official Synonym Symbols: RCH2; SRP1; IPOA5; NPI-1
• NCBI Protein Information: importin subunit alpha-5; SRP1-beta; importin alpha 5; importin-alpha-S1; RAG cohort protein 2; importin subunit alpha-1; nucleoprotein interactor 1; karyopherin subunit alpha-1; recombination activating gene cohort 2
• UniProt Protein Name: Importin subunit alpha-5
• UniProt Gene Name: KPNA1
• UniProt Synonym Gene Names: RCH2; NPI-1
• UniProt Entry Name: IMA5_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

KPNA1: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Belongs to the importin alpha family.

Protein type: Nuclear import; Karyopherin; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; cytoplasm; dendrite; nuclear pore; cytosol; nucleus

Molecular Function: protein binding; nuclear localization sequence binding; protein transporter activity

Biological Process: viral reproduction; positive regulation of protein import into nucleus; apoptosis; regulation of DNA recombination; cytokine and chemokine mediated signaling pathway; NLS-bearing substrate import into nucleus; DNA fragmentation during apoptosis; viral infectious cycle; cell structure disassembly during apoptosis