Typical Testing Data/Standard Curve (for reference only)
Mouse R-spondin 3 homolog (Xenopus laevis) ELISA Kit | RSPO3 elisa kit
Mouse R-spondin-3, RSPO3 ELISA Kit
Gene Names
Rspo3; Thsd2; AW742308; Cristin1; 2810459H04Rik
Reactivity
Mouse
Synonyms
R-spondin 3 homolog (Xenopus laevis); Mouse R-spondin-3; RSPO3 ELISA Kit; Mouse R-spondin-3 (RSPO3) ELISA kit; CRISTIN1; FLJ14440; PWTSR; THSD2; R-spondin 3; thrombospondin; type I; domain containing 2; thrombospondin-like; RSPO3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse RSPO3. No significant cross-reactivity or interference between mouse RSPO3 and analogues was observed.
Sequence Length
277
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
4.7 pg/ml -300 pg/ml.
Sensitivity
1.17 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RSPO3 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,454 Da
NCBI Official Full Name
R-spondin-3
NCBI Official Synonym Full Names
R-spondin 3 homolog (Xenopus laevis)
NCBI Official Symbol
Rspo3
NCBI Official Synonym Symbols
Thsd2; AW742308; Cristin1; 2810459H04Rik
NCBI Protein Information
R-spondin-3; cabriolet; cristin 1; cristin-1; nucleopondin; roof plate-specific spondin-3; thrombospondin, type I, domain 2; cysteine-rich and single thrombospondin domain-containing protein 1
UniProt Protein Name
R-spondin-3
Protein Family
UniProt Gene Name
Rspo3
UniProt Synonym Gene Names
Cristin-1
UniProt Entry Name
RSPO3_MOUSE
Uniprot Description
R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; frizzled binding; receptor binding
Biological Process: Wnt receptor signaling pathway; response to stimulus; angiogenesis
Research Articles on RSPO3
Similar Products
Product Notes
The Mouse RSPO3 rspo3 (Catalog #AAA937494) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA937494 ELISA Kit recognizes Mouse RSPO3. It is sometimes possible for the material contained within the vial of "R-spondin 3 homolog (Xenopus laevis), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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