4-1 BB Ligand Active Protein
4-1 BB Ligand, Recombinant, Human (4-1BBL, CD137L, TNFSF9, Tumor necrosis factor ligand superfamily member 9)
Purity
Molecular Biology Grade98% by SDS-PAGE and HPLC.
Synonyms
4-1 BB Ligand; Recombinant; Human (4-1BBL; CD137L; TNFSF9; Tumor necrosis factor ligand superfamily member 9); 4-1 BB Ligand active protein
Host
Recombinant, Human from E Coli
Purity/Purification
Molecular Biology Grade
98% by SDS-PAGE and HPLC.
98% by SDS-PAGE and HPLC.
Form/Format
The sterile filtered solution was lyophilized from 5mM Tris, pH 8.0, 75mM sodium chloride.
Biological Activity
Determined by the dose-dependent stimulation of IL-8 production by human PBMC (peripheral blood mononuclear cells). The ED50 for this effect is 5-10ng/ml.
Country of Origin
USA
Endotoxin Level
0.1ng/ug (1EU/ug)
Reconstitution
We recommend a quick spin followed by reconstitution in water to
a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
Preparation and Storage
-20 degree C
Stability: The Lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degree C. Reconstituted 4-1BBL should be storred in working aliquots at -20 degree C.
Stability: The Lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degree C. Reconstituted 4-1BBL should be storred in working aliquots at -20 degree C.
Related Product Information for 4-1 BB Ligand active protein
Human 4-1BB Ligand (4-1BBL, CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules.1-4 The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region.5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated Tcells, neurons and astrocytes.2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported.4 The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-) by monocytes.7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. Its seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4+ cells, and IFN- in CD8+ cells.9, 10
Product Categories/Family for 4-1 BB Ligand active protein
Similar Products
Product Notes
The 4-1 BB Ligand (Catalog #AAA650571) is an Active Protein produced from Recombinant, Human from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "4-1 BB Ligand, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
