4-1BBL Active Protein | TNFL9 active protein

4-1BBL, human recombinant

Cat. Num. AAA845403

• Gene Names: TNFSF9; CD137L; 4-1BB-L
• Applications: SDS-Page
• Purity: >=98%
• Synonyms: 4-1BBL; human recombinant; TNFSF9; CD137L; TNFL9 active protein

• Host: E Coli
• Purity/Purification: >=98%
• Form/Format: Lyophilized from 5 mM Tris, pH 8.0 + 75 mM NaCl. ; Appearance: Lyophilized protein
• Sequence Length: 254

• Applicable Applications for TNFL9 active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: We recommend a quick spin followed by reconstitution in H?O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
• Endotoxin Level: <0.1 ng/mug
• Biological Activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED?? for the effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.
• Results: 10 ng/ml
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for TNFL9 active protein

Background: Human 4-1BB Ligand (4-1BBL) is a member of the emerging family of Ligands with structural homology to tumor necrosis factor. Recombinant human 4-1BBL is a 19.5 kDa protein containing 185 amino acid residues, comprising the TNF-like extra-Cellular domain of 4-1BBL.

NCBI and Uniprot Product Information

• NCBI GI #: 4507609
• NCBI GeneID: 8744
• NCBI Accession #: NP_003802.1
• NCBI GenBank Nucleotide #: NM_003811.3
• UniProt Accession #: P41273; Q2M3S2
• Molecular Weight: 19.5 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 9
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 9
• NCBI Official Symbol: TNFSF9
• NCBI Official Synonym Symbols: CD137L; 4-1BB-L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 9
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 9
• UniProt Gene Name: TNFSF9
• UniProt Synonym Gene Names: 4-1BBL
• UniProt Entry Name: TNFL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008]

Uniprot Description

TNFL9: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Apoptosis; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; plasma membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; positive regulation of interferon-gamma production; positive regulation of cytotoxic T cell differentiation; cell-cell signaling; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 production; immune response; myeloid dendritic cell differentiation; signal transduction; positive regulation of activated T cell proliferation