Rat Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit | PDE3B elisa kit

Rat Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit

Cat. Num. AAA457127

• Reactivity: Rat
• Synonyms: Phosphodiesterase 3B; cGMP Inhibited (PDE3B); Rat Phosphodiesterase 3B; cGMP Inhibited (PDE3B) ELISA Kit; HcGIP1; cGIPDE1; CGIPDE1; CGIP1; CGI-PDE B; Cyclic GMP-inhibited phosphodiesterase B; PDE3B elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDE3B. No significant cross-reactivity or interference between PDE3B and analogues was observed.
• Sequence Length: 1108

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.123ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDE3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDE3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDE3B elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDE3B in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDE3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDE3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDE3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393929
• NCBI GeneID: 29516
• NCBI Accession #: NP_058925.1
• NCBI GenBank Nucleotide #: NM_017229.1
• UniProt Accession #: Q63085
• Molecular Weight: 123,107 Da
• NCBI Official Full Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• NCBI Official Synonym Full Names: phosphodiesterase 3B
• NCBI Official Symbol: Pde3b
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde3b
• UniProt Synonym Gene Names: CGI-PDE B

NCBI Description

catalyzes the hydrolysis of cAMP and cGMP; regulates insulin secretory processes [RGD, Feb 2006]

Uniprot Description

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis ().