Mouse Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit | PDE3B elisa kit

Mouse Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit

Cat. Num. AAA456909

• Gene Names: Pde3b; AI847709; 9830102A01Rik
• Reactivity: Mouse
• Synonyms: Phosphodiesterase 3B; cGMP Inhibited (PDE3B); Mouse Phosphodiesterase 3B; cGMP Inhibited (PDE3B) ELISA Kit; HcGIP1; cGIPDE1; CGIPDE1; CGIP1; CGI-PDE B; Cyclic GMP-inhibited phosphodiesterase B; PDE3B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDE3B. No significant cross-reactivity or interference between PDE3B and analogues was observed.
• Sequence Length: 1099

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: <18.75pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDE3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDE3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter- Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDE3B elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDE3B in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDE3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDE3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDE3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 112983648
• NCBI GeneID: 18576
• NCBI Accession #: NP_035185.2
• NCBI GenBank Nucleotide #: NM_011055.2
• UniProt Accession #: Q61409; Q8CIX5; Q9Z1J9
• Molecular Weight: 122,154 Da
• NCBI Official Full Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• NCBI Official Synonym Full Names: phosphodiesterase 3B, cGMP-inhibited
• NCBI Official Symbol: Pde3b
• NCBI Official Synonym Symbols: AI847709; 9830102A01Rik
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde3b
• UniProt Synonym Gene Names: CGI-PDE B

Uniprot Description

PDE3B: a cGMP-inhibited phosphodiesterase 1 involved in fat metabolism. Inhibition of PDE3B can augment PDE4 inhibitor-induced apoptosis in some patients with chronic lymphocytic leukemia.

Protein type: EC 3.1.4.17; Membrane protein, integral; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 7 F1|7 59.46 cM

Cellular Component: endoplasmic reticulum; Golgi apparatus; integral component of membrane; membrane

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cAMP binding; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; drug binding; hydrolase activity; metal ion binding; phosphoprotein binding; phosphoric diester hydrolase activity; protein kinase B binding

Biological Process: angiogenesis; cAMP catabolic process; cellular response to insulin stimulus; endocrine pancreas development; glucose homeostasis; negative regulation of angiogenesis; negative regulation of cell adhesion; negative regulation of insulin secretion; negative regulation of lipid catabolic process; regulation of insulin secretion; signal transduction