Testing Data
PDE3B recombinant protein
PDE3B, Active
Gene Names
PDE3B; HcGIP1; cGIPDE1
Applications
PDE Assay
Synonyms
PDE3B; Active; PDE3B recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
654-end
Sequence Length
6091
Applicable Applications for PDE3B recombinant protein
PDE Assay
Species
Human
Tag
GST tag
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for PDE3B recombinant protein
Description: Recombinant human PDE3B (654-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE3B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE3B has high affinity for cAMP and is inhibited by cGMP as well as specific inhibitors such as cilostamide and milrinone. PDE3B is phosphorylated and activated in response to insulin and agents that increase cAMP. Leptin can increase the activity of PDE3B in the hypothalamus leading to a decrease in cAMP concentration (1). PDE3B-knockout mice exhibited multiple alterations in regulation of lipolysis, lipogenesis, and insulin secretion, as well as signs of peripheral insulin resistance (2).
Scientific Background: PDE3B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE3B has high affinity for cAMP and is inhibited by cGMP as well as specific inhibitors such as cilostamide and milrinone. PDE3B is phosphorylated and activated in response to insulin and agents that increase cAMP. Leptin can increase the activity of PDE3B in the hypothalamus leading to a decrease in cAMP concentration (1). PDE3B-knockout mice exhibited multiple alterations in regulation of lipolysis, lipogenesis, and insulin secretion, as well as signs of peripheral insulin resistance (2).
Product Categories/Family for PDE3B recombinant protein
References
1. Zhao A Z, et al: A phosphatidylinositol 3-kinase-phosphodiesterase 3B-cyclic AMP pathway in hypothalamic action of leptin on feeding. Nature Neurosci. 5: 727-728, 2002. 2. Choi Y H, et al: Alterations in regulation of energy homeostasis in cyclic nucleotide phosphodiesterase 3B-null mice. J. Clin. Invest. 116: 3240-3251, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~85 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 3B (PDE3B), mRNA
NCBI Official Synonym Full Names
phosphodiesterase 3B
NCBI Official Symbol
PDE3B
NCBI Official Synonym Symbols
HcGIP1; cGIPDE1
NCBI Protein Information
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
UniProt Protein Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Protein Family
UniProt Gene Name
PDE3B
UniProt Synonym Gene Names
CGIP1; CGI-PDE B
Uniprot Description
PDE3B: a cGMP-inhibited phosphodiesterase 1 involved in fat metabolism. Inhibition of PDE3B can augment PDE4 inhibitor-induced apoptosis in some patients with chronic lymphocytic leukemia.
Protein type: EC 3.1.4.17; Membrane protein, integral; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Phosphodiesterase
Chromosomal Location of Human Ortholog: 11p15.2
Cellular Component: cytosol; endoplasmic reticulum; Golgi apparatus; membrane
Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; protein binding; protein kinase B binding
Biological Process: cAMP catabolic process; cellular response to insulin stimulus; negative regulation of angiogenesis; negative regulation of cell adhesion; negative regulation of cell adhesion mediated by integrin; negative regulation of lipid catabolic process
Research Articles on PDE3B
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Product Notes
The PDE3B pde3b (Catalog #AAA515629) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 654-end. AAA Biotech's PDE3B can be used in a range of immunoassay formats including, but not limited to, PDE Assay. Researchers should empirically determine the suitability of the PDE3B pde3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE3B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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