Glutaredoxin 1/GRX1 Recombinant Protein | GLRX recombinant protein

Glutaredoxin 1/GRX1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121696

• Gene Names: GLRX; GRX; GRX1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Glutaredoxin 1/GRX1; Glutaredoxin 1/GRX1 Protein; Human; Recombinant (His Tag); Human Glutaredoxin/GRX1/GLRX Protein (His Tag); GLRX Protein; GRX Protein; GRX1 Protein; glutaredoxin; GLRX recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tirs, 10% Glycerol, 1mM DTT, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln106

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human GLRX (P35754)(Met1-Gln106) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GLRX recombinant protein

Background: Glutaredoxin-1, also known as GRX1 and GLRX, belongs to theglutaredoxin family. Glutaredoxinsare smallredoxenzymes that useglutathioneas a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis ofdeoxyribonucleotidesby the enzymeribonucleotide reductase.

References

Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97. Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6. Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.

NCBI and Uniprot Product Information

• NCBI GI #: 169646776
• NCBI GeneID: 2745
• NCBI Accession #: NP_001112362.1
• NCBI GenBank Nucleotide #: NM_001118890.1
• UniProt Accession #: P35754; Q3KQS1; Q6ICT1; B2R4L2
• Molecular Weight: 11,776 Da
• NCBI Official Full Name: glutaredoxin-1
• NCBI Official Synonym Full Names: glutaredoxin (thioltransferase)
• NCBI Official Symbol: GLRX
• NCBI Official Synonym Symbols: GRX; GRX1
• NCBI Protein Information: glutaredoxin-1; TTase-1; thioltransferase-1
• UniProt Protein Name: Glutaredoxin-1
• UniProt Gene Name: GLRX
• UniProt Synonym Gene Names: GRX; TTase-1
• UniProt Entry Name: GLRX1_HUMAN

NCBI Description

This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

GLRX1: Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Belongs to the glutaredoxin family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 5q14

Cellular Component: mitochondrion; cytosol; nucleus

Molecular Function: electron carrier activity; protein N-terminus binding; glutathione disulfide oxidoreductase activity

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; cell redox homeostasis; positive regulation of membrane potential