Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit | PDE3A elisa kit

Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit

Cat. Num. AAA455773

• Gene Names: Pde3a; RNPDE3A
• Reactivity: Rat
• Synonyms: Phosphodiesterase 3A; cGMP Inhibited (PDE3A); Rat Phosphodiesterase 3A; cGMP Inhibited (PDE3A) ELISA Kit; CGI-PDE Cyclic GMP-inhibited phosphodiesterase A cGMP-inhibited 3'; 5'-cyclic phosphodiesterase A; PDE3A elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDE3A. No significant cross-reactivity or interference between PDE3A and analogues was observed.
• Sequence Length: 1141

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.27ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDE3A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDE3A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDE3A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDE3A in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDE3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDE3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8567398
• NCBI GeneID: 50678
• NCBI Accession #: NP_059033.1
• NCBI GenBank Nucleotide #: NM_017337.1
• UniProt Accession #: Q62865
• Molecular Weight: 124,301 Da
• NCBI Official Full Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• NCBI Official Synonym Full Names: phosphodiesterase 3A
• NCBI Official Symbol: Pde3a
• NCBI Official Synonym Symbols: RNPDE3A
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde3a
• UniProt Synonym Gene Names: CGI-PDE A

NCBI Description

plays a role in cyclic nucleotide degradation; may be involved in oocyte maturation [RGD, Feb 2006]

Uniprot Description

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.