Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit | PDE3A elisa kit

Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit

Cat. Num. AAA2019426

• Gene Names: PDE3A; HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
• Reactivity: Rat
• Synonyms: Phosphodiesterase 3A; cGMP Inhibited (PDE3A); Rat Phosphodiesterase 3A; cGMP Inhibited (PDE3A) ELISA Kit; CGI-PDE; Cyclic GMP-inhibited phosphodiesterase A; cGMP-inhibited 3'; 5'-cyclic phosphodiesterase A; PDE3A elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 3A, cGMP Inhibited (PDE3A).; No significant cross-reactivity or interference between Phosphodiesterase 3A, cGMP Inhibited (PDE3A) and analogues was observed.
• Sequence Length: 1141

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 3A, cGMP Inhibited (PDE3A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 3A, cGMP Inhibited (PDE3A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDE3A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 3A, cGMP Inhibited (PDE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 3A, cGMP Inhibited (PDE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 3A, cGMP Inhibited (PDE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 3A, cGMP Inhibited (PDE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PDE3A elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 119616812
• NCBI GeneID: 5139
• UniProt Accession #: Q14432; O60865; Q13348; Q17RD1
• Molecular Weight: 124,979 Da
• NCBI Official Full Name: phosphodiesterase 3A, cGMP-inhibited
• NCBI Official Synonym Full Names: phosphodiesterase 3A, cGMP-inhibited
• NCBI Official Symbol: PDE3A
• NCBI Official Synonym Symbols: HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE3A
• UniProt Synonym Gene Names: CGI-PDE A
• UniProt Entry Name: PDE3A_HUMAN

NCBI Description

This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

PDE3A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Inhibited by cGMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

Protein type: Membrane protein, integral; EC 3.1.4.17; Phosphodiesterase; Membrane protein, multi-pass; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: integral to membrane; cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; metal ion binding; cAMP binding

Biological Process: response to drug; diterpenoid metabolic process; cAMP-mediated signaling; response to cAMP; cGMP-mediated signaling; cAMP catabolic process; negative regulation of vascular permeability; regulation of meiosis; lipid metabolic process; blood coagulation; oocyte maturation; positive regulation of vascular permeability; negative regulation of apoptosis

Disease: Hypertension And Brachydactyly Syndrome