Human PROS1 ELISA Kit | PROS1 elisa kit

Human PROS1 PicoKine ELISA Kit

Cat. Num. AAA1751621

• Gene Names: PROS1; PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
• Reactivity: Human
• Synonyms: PROS1; Human PROS1 PicoKine ELISA Kit; Human PROS1; PROS1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Proc
• Sequence Length: 40

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta) And Urine
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: <0.1ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PROS1 elisa kit

Principle of the Assay: The Picokine Human Proc Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human Proc with a 96-well strip plate that is pre-coated with antibody specific for Proc. The detection antibody is a biotinylated antibody specific for Proc. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human Proc with immunogen: Expression system for standard: NSO, Immunogen sequence: A43-P461. The kit is analytically validated with ready to use reagents. To measure Human Proc, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human Proc in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human Proc in the sample!!Background/Introduction: Protein C(PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

NCBI and Uniprot Product Information

• NCBI GI #: 27597166
• NCBI GeneID: 5627
• NCBI Official Full Name: PROS1, partial
• NCBI Official Synonym Full Names: protein S
• NCBI Official Symbol: PROS1
• NCBI Official Synonym Symbols: PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
• NCBI Protein Information: vitamin K-dependent protein S
• Protein Family: Proline--tRNA ligase

NCBI Description

This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]