Mouse Gp130/IL6ST ELISA Kit | IL6ST elisa kit

Mouse Gp130/IL6ST PicoKine Fast ELISA Kit

Cat. Num. AAA1752737

• Gene Names: Il6st; CD130; gp130; AA389424; BB405851; D13Ertd699e; 5133400A03Rik
• Reactivity: Mouse
• Synonyms: Gp130/IL6ST; Mouse Gp130/IL6ST PicoKine Fast ELISA Kit; Mouse Gp130; Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st; IL6ST elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Il6st
• Sequence Length: 917

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA, citrate)
• Detection Range: 125pg/ml-8000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL6ST elisa kit

Principle of the Assay: The Picokine™ Mouse Il6st Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Il6st with a 96-well strip plate that is pre-coated with antibody specific for Il6st. The detection antibody is a biotinylated antibody specific for Il6st. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il6st with immunogen: Expression system for standard: NSO; Immunogen sequence: Q23-E617. The kit is analytically validated with ready to use reagents. To measure Mouse Il6st, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Il6st in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il6st in the sample.!!Background/Introduction: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

NCBI and Uniprot Product Information

• NCBI GI #: 225007625
• NCBI GeneID: 16195
• NCBI Accession #: NP_034690.3
• NCBI GenBank Nucleotide #: NP_034690.3
• UniProt Accession #: Q00560
• NCBI Official Full Name: interleukin-6 receptor subunit beta
• NCBI Official Synonym Full Names: interleukin 6 signal transducer
• NCBI Official Symbol: Il6st
• NCBI Official Synonym Symbols: CD130; gp130; AA389424; BB405851; D13Ertd699e; 5133400A03Rik
• NCBI Protein Information: interleukin-6 receptor subunit beta
• UniProt Protein Name: Interleukin-6 receptor subunit beta
• Protein Family: Interleukin-6 receptor
• UniProt Gene Name: Il6st
• UniProt Synonym Gene Names: IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130
• UniProt Entry Name: IL6RB_MOUSE

Uniprot Description

gp130: gp130 is a ubiquitously expressed type I cytokine family receptor. The receptor systems for IL6, LIF, OSM, CNTF, IL11 AND CT1 utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not directly bind IL6. May have a role in embryonic development. Contains 5 fibronectin type III domains and 1 immunoglobulin-like C2-type domain. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: cell soma; dendrite; external side of plasma membrane; interleukin-6 receptor complex; membrane; oncostatin-M receptor complex

Molecular Function: ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding; growth factor binding; interleukin-11 binding; interleukin-11 receptor activity; interleukin-6 binding; interleukin-6 receptor activity; interleukin-6 receptor binding; leukemia inhibitory factor receptor activity; oncostatin-M receptor activity; protein binding; protein heterodimerization activity

Biological Process: cytokine and chemokine mediated signaling pathway; glycogen metabolic process; leukemia inhibitory factor signaling pathway; positive regulation of astrocyte differentiation; positive regulation of cell proliferation; positive regulation of osteoblast differentiation; positive regulation of smooth muscle cell migration; positive regulation of T cell proliferation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; reduction of cytosolic calcium ion concentration; regulation of Notch signaling pathway; response to cytokine stimulus; signal transduction; triacylglycerol mobilization