PLK1 Polyclonal Antibody | anti-PLK1 antibody

Anti-PLK1 Antibody

Cat. Num. AAA178251

• Gene Names: PLK1; PLK; STPK13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: PLK1; Polyclonal Antibody; Anti-PLK1 Antibody; Serine/threonine-protein kinase PLK1; Cell cycle regulated protein kinase; PLK 1; PLK; PLK-1; plk1; PLK1_HUMAN; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase 13; Serine/threonine protein kinase PLK1; Serine/threonine-protein kinase 13; STPK 13; STPK13; polo-like kinase 1; anti-PLK1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 603

• Applicable Applications for anti-PLK1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- PLK1 Picoband antibody, MBS178251, Western blottingAll lanes: Anti PLK1 (MBS178251) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD)

Immunohistochemistry (IHC)

(Anti- PLK1 Picoband antibody, MBS178251,IHC(P)IHC(P): Mouse Lymphaden Tissue )

Immunohistochemistry (IHC)

(Anti- PLK1 Picoband antibody, MBS178251,IHC(P)IHC(P): Rat Lymphaden Tissue )

Immunohistochemistry (IHC)

(Anti- PLK1 Picoband antibody, MBS178251,IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-PLK1 antibody

Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase PLK1(PLK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13(STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

References

1. Holtrich U, Wolf G, Bräuninger A, Karn T, Böhme B, Rübsamen-Waigmann H et al. (March 1994). "Induction and down-regulation of PLK, a human serine/threonine kinase expressed in proliferating cells and tumors". Proc. Natl. Acad. Sci. U.S.A. 91(5): 1736-40. 2. Soung NK, Park JE, Yu LR, Lee KH, Lee JM, Bang JK et al. (April 2009). "Plk1-dependent and -independent roles of an ODF2 splice variant, hCenexin1, at the centrosome of somatic cells". Dev. Cell 16(4): 539-50.

NCBI and Uniprot Product Information

• NCBI GI #: 21359873
• NCBI GeneID: 5347
• NCBI Accession #: NP_005021.2
• NCBI GenBank Nucleotide #: NM_005030.5
• UniProt Accession #: P53350; Q15153; Q99746
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13
• UniProt Entry Name: PLK1_HUMAN

NCBI Description

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.

Protein type: Protein kinase, Other; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: centrosome; chromatin; cytoplasm; cytosol; kinetochore; microtubule cytoskeleton; midbody; nucleolus; nucleoplasm; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole; synaptonemal complex

Molecular Function: ATP binding; kinase activity; microtubule binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: anaphase-promoting complex activation during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; centrosome organization and biogenesis; cytokinesis; cytokinesis after mitosis; establishment of protein localization; female meiosis chromosome segregation; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; homologous chromosome segregation; microtubule bundle formation; mitosis; mitotic cell cycle spindle assembly checkpoint; mitotic nuclear envelope disassembly; mitotic sister chromatid segregation; negative regulation of apoptosis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of proteolysis; positive regulation of ubiquitin-protein ligase activity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein amino acid phosphorylation; protein destabilization; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cell cycle; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; regulation of protein binding; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion