Rat IGF2 ELISA Kit | IGF2 elisa kit

Rat IGF2 ELISA Kit

Cat. Num. AAA824828

• Gene Names: Igf2; IGFII; RNIGF2
• Reactivity: Rat
• Applications: ELISA
• Synonyms: IGF2; Rat IGF2 ELISA Kit; Insulin-like growth factor II; IGF-II; Somatomedin-A; IGF2 elisa kit

• Reactivity: Rat
• Specificity: The Rat IGF2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IGF2 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 180

• Applicable Applications for IGF2 elisa kit: Sandwich ELISA (SE)
• Samples: Cell culture supernatants, serum, plasma
• Sensitivity: < 5 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF2 elisa kit

Principle of the Assay: The MyBioSource' Rat IGF2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IGF2 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

NCBI and Uniprot Product Information

• NCBI GI #: 297747332
• NCBI GeneID: 24483
• NCBI Accession #: NP_001177091.1
• NCBI GenBank Nucleotide #: NM_001190162.1
• UniProt Accession #: P01346
• Molecular Weight: 20,086 Da
• NCBI Official Full Name: insulin-like growth factor II isoform 2 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 2
• NCBI Official Symbol: Igf2
• NCBI Official Synonym Symbols: IGFII; RNIGF2
• NCBI Protein Information: insulin-like growth factor II; MSA; IGF-II; multiplication-stimulating activity; insulin-like growth factor II (IGF-II); multiplication-stimulating polypeptide; Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein
• UniProt Protein Name: Insulin-like growth factor II
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf2
• UniProt Synonym Gene Names: Igf-2; IGF-II; MSA
• UniProt Entry Name: IGF2_RAT

NCBI Description

a mitogenic growth factor; may have a role in fetal development [RGD, Feb 2006]

Uniprot Description

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.