Typical Testing Data/Standard Curve (for reference only)
(Rat IGF-2 PicoKine ELISA Kit standard curve )
Rat IGF-2 ELISA Kit | IGF2 elisa kit
Rat IGF-2 PicoKine ELISA Kit
Gene Names
Igf2; IGFII; RNIGF2
Reactivity
Rat
Synonyms
IGF-2; Rat IGF-2 PicoKine ELISA Kit; Insulin-like growth factor II; insulin-like growth factor 2 (somatomedin A); C11orf43; FLJ22066; FLJ44734; IGF 2; IGF II; IGF-II; IGF2; IGF2_HUMAN; IGFII; INSIGF; Insulin like Growth Factor 2; Insulin like growth factor 2 (somatomedin A); Insulin like growth factor II; Insulin like growth factor II precursor; Insulin like growth factor type 2; pp9974; Preptin; Putative insulin like growth factor II associated protein; Somatomedin A; Somatomedin-A; IGF2 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat IGF-2
Sequence Length
180
Samples
Cell culture supernates, serum and plasma(heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<5pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat IGF-2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat IGF-2 PicoKine ELISA Kit standard curve )
Related Product Information for IGF2 elisa kit
Principle of the Assay: The rat IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-2 has been precoated onto 90-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biolin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-2 amount of sample captured in plate.
Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. 1 It is paternally expressed in the fetus and placenta. 2 IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele irherited from the father is active and the allele inhedted from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5
Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. 1 It is paternally expressed in the fetus and placenta. 2 IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele irherited from the father is active and the allele inhedted from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
20,086 Da
NCBI Official Full Name
Insulin-like growth factor II
NCBI Official Synonym Full Names
insulin-like growth factor 2
NCBI Official Symbol
Igf2
NCBI Official Synonym Symbols
IGFII; RNIGF2
NCBI Protein Information
insulin-like growth factor II; MSA; IGF-II; multiplication-stimulating activity; insulin-like growth factor II (IGF-II); multiplication-stimulating polypeptide; Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein
UniProt Protein Name
Insulin-like growth factor II
Protein Family
UniProt Gene Name
Igf2
UniProt Synonym Gene Names
Igf-2; IGF-II; MSA
UniProt Entry Name
IGF2_RAT
NCBI Description
a mitogenic growth factor; may have a role in fetal development [RGD, Feb 2006]
Uniprot Description
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Research Articles on IGF2
Similar Products
Product Notes
The Rat IGF2 igf2 (Catalog #AAA175899) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175899 ELISA Kit recognizes Rat IGF2. It is sometimes possible for the material contained within the vial of "IGF-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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