Mouse Mast cell protease 8 ELISA Kit | Mcpt8 elisa kit

Mouse Mast cell protease 8 ELISA Kit

Cat. Num. AAA761748

• Gene Names: Mcpt8; MMCP-8; BB139338
• Reactivity: Mouse
• Synonyms: Mast cell protease 8; Mouse Mast cell protease 8 ELISA Kit; Mcpt8/Mast cell protease 8/mMCP-8; Mcpt8 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mcpt4. No significant cross-reactivity or interference between Mcpt4 and analogues was observed.
• Sequence Length: 247

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 46.875-3000pg/ml
• Sensitivity: <28.125pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mcpt4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mcpt4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Mcpt8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mcpt4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mcpt4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mcpt4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mcpt4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6678840
• NCBI GeneID: 17231
• NCBI Accession #: NP_032598.1
• NCBI GenBank Nucleotide #: NM_008572.1
• Molecular Weight: 27,122 Da
• NCBI Official Full Name: mast cell protease 8
• NCBI Official Synonym Full Names: mast cell protease 8
• NCBI Official Symbol: Mcpt8
• NCBI Official Synonym Symbols: MMCP-8; BB139338
• NCBI Protein Information: mast cell protease 8
• UniProt Protein Name: Mast cell protease 8
• Protein Family: Mast cell protease
• UniProt Gene Name: Mcpt8
• UniProt Synonym Gene Names: mMCP-8
• UniProt Entry Name: MCPT8_MOUSE

Uniprot Description

Mcpt8:

Cellular Component: cytoplasm; extracellular region; intracellular membrane-bound organelle

Molecular Function: hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: immune response; protein processing; proteolysis