Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Galectin-4 (LGALS4) Standard Curve.)
Rat Galectin-4 (LGALS4) ELISA Kit | LGALS4 elisa kit
Rat Galectin-4 (LGALS4) ELISA Kit
Gene Names
Lgals4; L-36; L36LBI
Reactivity
Rat
Synonyms
Galectin-4 (LGALS4); Rat Galectin-4 (LGALS4) ELISA Kit; LGALS4; GAL4; L36LBP; L-36 lactose-binding protein; galectin 4; galectin-4; lactose-binding lectin 4; LGALS4 elisa kit
Reactivity
Rat
Specificity
Rat Galectin-4 (LGALS4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LGALS4. No significant cross-reactivity or interference between Rat LGALS4 and analogues was observed.
Sequence Length
324
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Galectin-4 (LGALS4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Galectin-4 (LGALS4) Standard Curve.)
Related Product Information for LGALS4 elisa kit
Description: This Rat Galectin-4 (LGALS4) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS4 in samples. An antibody specific for LGALS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical. Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different. Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands. Similarly to other galectins, galectin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine.
Background: Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical. Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different. Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands. Similarly to other galectins, galectin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
galectin-4
NCBI Official Synonym Full Names
galectin 4
NCBI Official Symbol
Lgals4
NCBI Official Synonym Symbols
L-36; L36LBI
NCBI Protein Information
galectin-4
UniProt Protein Name
Galectin-4
Protein Family
UniProt Gene Name
Lgals4
UniProt Synonym Gene Names
Gal-4; L36LBP
NCBI Description
lactose-binding lectin [RGD, Feb 2006]
Uniprot Description
Galectin that binds lactose and a related range of sugars.
Research Articles on LGALS4
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Product Notes
The Rat LGALS4 lgals4 (Catalog #AAA9712366) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712366 ELISA Kit recognizes Rat LGALS4. It is sometimes possible for the material contained within the vial of "Galectin-4 (LGALS4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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