Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Galectin-12 (LGALS12) Standard Curve.)
Human Galectin-12 (LGALS12) ELISA Kit | LGALS12 elisa kit
Human Galectin-12 (LGALS12) ELISA Kit
Gene Names
LGALS12; GAL12; GRIP1
Reactivity
Human
Synonyms
Galectin-12 (LGALS12); Human Galectin-12 (LGALS12) ELISA Kit; LGALS12; GALECTIN-12; GRIP1; galectin 12; LGALS12 elisa kit
Reactivity
Human
Specificity
Human Galectin-12 (LGALS12) ELISA Kit has high sensitivity and excellent specificity for detection of Human LGALS12. No significant cross-reactivity or interference between Human LGALS12 and analogues was observed.
Sequence Length
266
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Galectin-12 (LGALS12) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Galectin-12 (LGALS12) Standard Curve.)
Related Product Information for LGALS12 elisa kit
Description: This Human Galectin-12 (LGALS12) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS12 in samples. An antibody specific for LGALS12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Galectins constitute a family of proteins that bind to beta-galactoside residues and have diverse physiological functions. Report on the identification of a galectin-like molecule, galectin-12, in a human adipose tissue cDNA library. The protein contained two potential carbohydrate-recognition domains with the second carbohydrate-recognition domain being less conserved compared with other galectins. Galectin-12 mRNA was predominantly expressed in adipose tissue of human and mouse and in differentiated 3T3-L1 adipocytes. Caloric restriction and treatment of obese animals with troglitazone increased galectin-12 mRNA levels and decreased the average size of the cells in adipose tissue.
Background: Galectins constitute a family of proteins that bind to beta-galactoside residues and have diverse physiological functions. Report on the identification of a galectin-like molecule, galectin-12, in a human adipose tissue cDNA library. The protein contained two potential carbohydrate-recognition domains with the second carbohydrate-recognition domain being less conserved compared with other galectins. Galectin-12 mRNA was predominantly expressed in adipose tissue of human and mouse and in differentiated 3T3-L1 adipocytes. Caloric restriction and treatment of obese animals with troglitazone increased galectin-12 mRNA levels and decreased the average size of the cells in adipose tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
galectin-12 isoform 5
NCBI Official Synonym Full Names
galectin 12
NCBI Official Symbol
LGALS12
NCBI Official Synonym Symbols
GAL12; GRIP1
NCBI Protein Information
galectin-12
UniProt Protein Name
Galectin-12
Protein Family
UniProt Gene Name
LGALS12
UniProt Synonym Gene Names
GRIP1; Gal-12
UniProt Entry Name
LEG12_HUMAN
NCBI Description
This gene encodes a member of the galectin superfamily, a group of beta-galactoside-binding proteins with conserved carbohydrate recognition domains. The related mouse protein is a primary regulator of the early stages of adipose tissue development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]
Uniprot Description
LGALS12: Binds lactose. May participate in the apoptosis of adipocytes. 6 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: mitochondrion; nucleus
Molecular Function: lactose binding
Research Articles on LGALS12
Similar Products
Product Notes
The Human LGALS12 lgals12 (Catalog #AAA9715488) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715488 ELISA Kit recognizes Human LGALS12. It is sometimes possible for the material contained within the vial of "Galectin-12 (LGALS12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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