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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Galectin 3 (GAL-3) Standard Curve.)

Mouse Galectin 3 (GAL-3) ELISA Kit | GAL-3 elisa kit

Mouse Galectin 3 (GAL-3) ELISA Kit

Gene Names
Lgals3; GBP; L-34; gal3; Mac-2
Reactivity
Mouse
Synonyms
Galectin 3 (GAL-3); Mouse Galectin 3 (GAL-3) ELISA Kit; LGALS3; CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; galectin 3; galectin-3 internal; laminin-binding protein; GAL-3 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse Galectin 3 (GAL-3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LGALS3. No significant cross-reactivity or interference between Mouse LGALS3 and analogues was observed.
Sequence Length
264
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Galectin 3 (GAL-3) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Galectin 3 (GAL-3) Standard Curve.)
Related Product Information for GAL-3 elisa kit
Description: This Mouse Galectin 3 (GAL-3) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS3 in samples. An antibody specific for LGALS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Galectin-3 contains a carboxyl-terminal lectin domain and an amino-terminal non-lectin part consisting primarily of short tandem repeats. It is widely distributed in tissues and found in epithelial cells, fibroblasts, dendritic cells, and inflammatory cells. In many cell types studied, galectin-3 is present diffusely in the cytoplasm, but is also localized to the nucleus and subcellular structures, such as mitochondria, phagosomes and exosomes, under specific conditions. It is secreted by various cell types, including monocytes, macrophages, and epithelial cells, and the extracellular protein can bind to a large number of different glycoconjugates on the cell surfaces and extracellular matrices.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Galectin-3
NCBI Official Synonym Full Names
lectin, galactose binding, soluble 3
NCBI Official Symbol
Lgals3
NCBI Official Synonym Symbols
GBP; L-34; gal3; Mac-2
NCBI Protein Information
galectin-3
UniProt Protein Name
Galectin-3
UniProt Gene Name
Lgals3
UniProt Synonym Gene Names
Gal-3; CBP 35
UniProt Entry Name
LEG3_MOUSE

Uniprot Description

galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Extracellular matrix; Cell surface; Motility/polarity/chemotaxis

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; extracellular matrix; extracellular region; extracellular space; immunological synapse; membrane; mitochondrial inner membrane; nucleus; proteinaceous extracellular matrix; spliceosome

Molecular Function: advanced glycation end-product receptor activity; carbohydrate binding; chemoattractant activity; disaccharide binding; IgE binding; laminin binding; monosaccharide binding; protein binding

Biological Process: cell differentiation; eosinophil chemotaxis; extracellular matrix organization and biogenesis; immune system process; innate immune response; macrophage chemotaxis; maintenance of protein localization; monocyte chemotaxis; mRNA processing; negative regulation of apoptosis; negative regulation of endocytosis; negative regulation of T cell receptor signaling pathway; neutrophil chemotaxis; positive chemotaxis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of serotonin secretion; regulation of T cell proliferation; RNA splicing; signal transduction; skeletal development

Research Articles on GAL-3

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Product Notes

The Mouse GAL-3 lgals3 (Catalog #AAA9717791) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717791 ELISA Kit recognizes Mouse GAL-3. It is sometimes possible for the material contained within the vial of "Galectin 3 (GAL-3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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