Rabbit anti-Human RFWD2 Polyclonal Antibody | anti-RFWD2 antibody

RFWD2, CT (RFWD2, COP1, RNF200, E3 ubiquitin-protein ligase RFWD2, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200) (APC)

Cat. Num. AAA6341511

• Gene Names: RFWD2; COP1; RNF200
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RFWD2; Polyclonal Antibody; CT (RFWD2; COP1; RNF200; E3 ubiquitin-protein ligase RFWD2; Constitutive photomorphogenesis protein 1 homolog; RING finger and WD repeat domain protein 2; RING finger protein 200) (APC); RING finger protein 200; anti-RFWD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RFWD2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RFWD2 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RFWD2 antibody detected the RFWD2 protein (arrow).)

Related Product Information for anti-RFWD2 antibody

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

Product Categories/Family for anti-RFWD2 antibody

Antibodies; Ubiquitin Ligases

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Kinyo, A., et al. J. Invest. Dermatol. 130(2):541-545(2010). Li, D.Q., et al. Proc. Natl. Acad. Sci. U.S.A. 106(41):17493-17498(2009). Kato, S., et al. J. Biol. Chem. 283(51):35464-35473(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 21359963
• NCBI GeneID: 64326
• NCBI Accession #: NP_071902.2
• NCBI GenBank Nucleotide #: NM_022457.6
• UniProt Accession #: Q8NHY2; Q504W6; Q6H103; Q9H6L7; E9PKI0; X5D9B4
• Molecular Weight: 14,914 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RFWD2 isoform a
• NCBI Official Synonym Full Names: ring finger and WD repeat domain 2
• NCBI Official Symbol: RFWD2
• NCBI Official Synonym Symbols: COP1; RNF200
• NCBI Protein Information: E3 ubiquitin-protein ligase RFWD2
• UniProt Protein Name: E3 ubiquitin-protein ligase RFWD2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RFWD2
• UniProt Synonym Gene Names: COP1; RNF200; hCOP1
• UniProt Entry Name: RFWD2_HUMAN

Uniprot Description

COP1: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Homodimer. Homodimerization is mediated by the coiled coil domain. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Isoform 2 does not interact with CUL4A but still binds to RBX1, suggesting that the interaction may be mediated by another culllin protein. Isoform 1 and isoform 2 interact with CUL5 but not with CUL1, CUL2 not CUL3. Interacts with bZIP transcription factors JUN, JUNB and JUND but not with FOS, ATF2 nor XBP1. Interacts with p53 (TP53). By p53/TP53. Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. Belongs to the COP1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 1q25.1-q25.2

Cellular Component: Golgi membrane; nucleoplasm; centrosome; focal adhesion; cytoplasm; nuclear speck; cytosol

Molecular Function: protein binding; zinc ion binding; ligase activity

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; response to ionizing radiation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest