Rat FATP4 (FATP4) ELISA Kit | FATP4 elisa kit

Rat FATP4 (FATP4) ELISA Kit

Cat. Num. AAA7606148

• Gene Names: Slc27a4; FATP4; BB144259
• Reactivity: Rat
• Synonyms: FATP4 (FATP4); Rat FATP4 (FATP4) ELISA Kit; FATP4 elisa kit

• Reactivity: Rat
• Sequence Length: 506

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FATP4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FATP4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FATP4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FATP4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FATP4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 3335569
• NCBI GeneID: 26569
• Molecular Weight: 72,319 Da
• NCBI Official Full Name: fatty acid transport protein 4, partial
• NCBI Official Synonym Full Names: solute carrier family 27 (fatty acid transporter), member 4
• NCBI Official Symbol: Slc27a4
• NCBI Official Synonym Symbols: FATP4; BB144259
• NCBI Protein Information: long-chain fatty acid transport protein 4
• UniProt Protein Name: Long-chain fatty acid transport protein 4
• UniProt Gene Name: Slc27a4
• UniProt Synonym Gene Names: Acsvl4; Fatp4; FATP-4; Fatty acid transport protein 4

Uniprot Description

SLC27A4: Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. Defects in SLC27A4 are the cause of ichthyosis prematurity syndrome (IPS). A keratinization disorder characterized by complications in the second trimester of pregnancy resulting from polyhydramnion, with premature birth of a child with thick caseous desquamating epidermis, respiratory complications and transient eosinophilia. After recovery during the first months of life, the symptoms are relatively benign and the patients suffer from a lifelong non-scaly ichthyosis with atopic manifestations. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: EC 6.2.1.-; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 2 B|2 20.64 cM

Cellular Component: brush border membrane; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; microvillus; plasma membrane

Molecular Function: long-chain-fatty-acid-CoA ligase activity; very-long-chain-fatty-acid-CoA ligase activity

Biological Process: fatty acid metabolic process; long-chain fatty acid metabolic process; long-chain fatty acid transport; medium-chain fatty acid transport; skin development; very-long-chain fatty acid catabolic process; very-long-chain fatty acid metabolic process