Rat Estradiol ELISA Kit
Estradiol (Rat) ELISA
Reactivity
Rat
Synonyms
Estradiol; Estradiol (Rat) ELISA; Estradiol elisa kit
Reactivity
Rat
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is > 90 %.
Samples
Human Serum
Preparation and Storage
2 - 8 degree C
Related Product Information for Estradiol elisa kit
Intended Uses: The MyBioSource ELISA is intended for the determination of IgM class antibodies against Coxiella burnetii phase 2 in the early stages of infection in human serum. For Research Use Only.
Principle of the Assay: The immunoenzymatic determination of IgM-class antibodies to Coxiella burnetii is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with phase 2 antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Coxiella burnetii-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of phase 2-specific IgM antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader!!Background/Introduction: Q-Fever is a disease that results from infection with small, polymorph and gramnegative bacteria called Coxiella burnetii. After an outbreak in Brisbane, Australia, the responsible organism was isolated and named Coxiella burnetii in honour of Dr. Herald Rae Cox and Sir Frank Burnet. New molecular research demonstrated a close relationship to Legionella. The zoonosis Q-Fever is found everywhere except New Zealand (no data available). There is an extensive reservoir (mainly ticks) of C. burnetii. Ticks are an important vector of the pathogen in the transmission between domestic and wildlife animals. But the ticks are unimportant in the direct infection of humans. Cattles, sheep and goats are usually the source of transmission of this microorganism to humans. However cats, dogs and rabbits are also important in this regard. In most instances humans become infected with Coxiella burnetii following inhalation of contaminated aerosols (respiratory tract). The incubation period for QFever in humans is about 2 weeks. The resulting illness can be divided into acute and chronic varieties. During the acute phase of illness antibodies to the phase IIantigen are formed. Anti phase-I antibodies in high titers are typical for a chronic disease. In areas where Q-Fever is endemic, 12% or more of the population have antibodies to C. burnetii. Most of the infections are subclinical or unidentified. The acute infection shows symptoms of high fever, shivers, muscle pain and headache. Later on more severe diseases such as pneumonia or hepatitis can occur. Infections during pregnancy can lead to an abort or premature birth. Approximately 1% of all infections become chronic. The most frequent organ manifestation in Q-Fever is endocarditis.
Principle of the Assay: The immunoenzymatic determination of IgM-class antibodies to Coxiella burnetii is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with phase 2 antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Coxiella burnetii-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of phase 2-specific IgM antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader!!Background/Introduction: Q-Fever is a disease that results from infection with small, polymorph and gramnegative bacteria called Coxiella burnetii. After an outbreak in Brisbane, Australia, the responsible organism was isolated and named Coxiella burnetii in honour of Dr. Herald Rae Cox and Sir Frank Burnet. New molecular research demonstrated a close relationship to Legionella. The zoonosis Q-Fever is found everywhere except New Zealand (no data available). There is an extensive reservoir (mainly ticks) of C. burnetii. Ticks are an important vector of the pathogen in the transmission between domestic and wildlife animals. But the ticks are unimportant in the direct infection of humans. Cattles, sheep and goats are usually the source of transmission of this microorganism to humans. However cats, dogs and rabbits are also important in this regard. In most instances humans become infected with Coxiella burnetii following inhalation of contaminated aerosols (respiratory tract). The incubation period for QFever in humans is about 2 weeks. The resulting illness can be divided into acute and chronic varieties. During the acute phase of illness antibodies to the phase IIantigen are formed. Anti phase-I antibodies in high titers are typical for a chronic disease. In areas where Q-Fever is endemic, 12% or more of the population have antibodies to C. burnetii. Most of the infections are subclinical or unidentified. The acute infection shows symptoms of high fever, shivers, muscle pain and headache. Later on more severe diseases such as pneumonia or hepatitis can occur. Infections during pregnancy can lead to an abort or premature birth. Approximately 1% of all infections become chronic. The most frequent organ manifestation in Q-Fever is endocarditis.
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Product Notes
The Rat Estradiol (Catalog #AAA495068) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495068 ELISA Kit recognizes Rat Estradiol. It is sometimes possible for the material contained within the vial of "Estradiol, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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