Typical Testing Data/Standard Curve (for reference only)
Canine TSH ELISA Kit | TSH elisa kit
TSH (Canine) ELISA
Reactivity
Canine
Synonyms
TSH; TSH (Canine) ELISA; TSH elisa kit
Reactivity
Canine
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the spe-cific analyte. (Detected by method comparison with Mikrogen ELISA, with three lots of MyBioSource' ELISA. 77 samples, therefrom 57 negative samples are assayed with MyBioSource' ELISA lot 1-3.) It is 100% (for all three lots).
Sequence Length
1377
Samples
Human Serum And Plasma
Assay Type
Competitive
Detection Range
0.52-60 DU/mL.
Intra-assay Precision
The intra-assay (within-run) precision of the MyBioSource' Treponema pallidum IgM ELISA was deter-mined by 20 x measurements of 12 serum samples covering the whole measuring range
Inter-assay Precision
The inter-assay variation of the MyBioSource pallidum IgM ELISA was determined with 3 samples with 2 production kits in 10 independent runs with 2 replicates per run.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TSH elisa kit
Intended Uses: The MyBioSource' Treponema pallidum IgM Enzyme Immunoassay Kit provides materials for the determination of IgM-class antibodies to Treponema pallidum in human serum and plasma.
Principle of the Assay: The MyBioSource' Treponema pallidum IgM ELISA Kit is a solid phase enzyme-linked immuno-sorbent assay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pre-treatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Treponema pallidum antigen. Pretreated samples and ready-for-use controls are pipetted into these wells. During incubation Treponema pallidum-specific antibodies of positive samples and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conju-gated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked im-mune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Treponema pallidum-specific IgM an-tibody in the sample. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
Principle of the Assay: The MyBioSource' Treponema pallidum IgM ELISA Kit is a solid phase enzyme-linked immuno-sorbent assay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pre-treatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Treponema pallidum antigen. Pretreated samples and ready-for-use controls are pipetted into these wells. During incubation Treponema pallidum-specific antibodies of positive samples and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conju-gated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked im-mune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Treponema pallidum-specific IgM an-tibody in the sample. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
Product Categories/Family for TSH elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
148,227 Da
NCBI Official Full Name
Tsh
UniProt Protein Name
Temperature-sensitive hemagglutinin tsh autotransporter
Protein Family
UniProt Gene Name
tsh
UniProt Entry Name
TSH_ECOLX
Uniprot Description
Contributes to the development of lesions and deposition of fibrin in the avian air sacs. It can act both as an adhesin and as a serine protease. Agglutinates erythrocytes while in contact with the extracellular surface of the bacterial cells. Can adhere to purified hemoglobin and bind with great efficiency to extracellular matrix proteins. Cleaves casein and exhibits mucinolytic activity.
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Product Notes
The Canine TSH tsh (Catalog #AAA495053) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495053 ELISA Kit recognizes Canine TSH. It is sometimes possible for the material contained within the vial of "TSH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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