Typical Testing Data/Standard Curve (for reference only)
BAFF elisa kit
BAFF ELISA Kit
Synonyms
BAFF; BAFF ELISA Kit; B-cell activating factor; BAFF elisa kit
Sequence Length
264
Samples
Faecal Samples
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BAFF elisa kit
Intended Uses: The Astrovirus ELISA is an enzyme immunoassay for the measurement of Astrovirus in faecal samples. For Research Use Only..
Principle of the Assay: The Astrovirus is a one-step enzyme immunoassay on the basis of polyclonal and monoclonal antibodies against Astrovirus antigens. Diluted stool specimens and horseradish peroxidase (HRP) labelled monoclonal anti-Astrovirus-antibodies are dispensed simultaneously into the wells of a microtitration plate coated with polyclonal anti-Astrovirus-antibodies. After an incubation time of 60 min at room temperature (RT) unbound components are removed by a washing step. HRP converts the subsequently added colourless substrate solution of 3,3',5,5'-Tetramethylbenzidine (TMB) within a 10 min reaction time at room temperature protected from light into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 / >= 620 nm is directly proportional to the specifically bound amount of Astrovirus!!Background/Introduction: Astrovirus was firstly described in 1975 and named according to its star-shaped structure visible under the electron microscope. Astrovirus belongs to the family Astroviridae. Human Astroviruses are subdivided into 7 serotypes (1). Together with Rotavirus and Adenovirus Astrovirus is one of the most common causes of non-bacterial gastroenteritis in children under 5 years of age all over the world. Thus 80% of children between 5 and 10 years of age are anti-Astrovirus-antibody positive. Astrovirus caused gastroenteritis in adults and nosocomial infections are observed as well (2). The course of the disease is usually self-limiting and of short duration. After the incubation time of 1-2 days a 1-4 days lasting gastroenteritis develops accompanied by vomiting, diarrhea, fever and abdominal pain finally causing dehydration. Although occurring all over the year Astrovirus infections are mainly observed during the winter months (3, 4). Astrovirus infections are spread via faecal-oral transmission from person to person or via contaminated things or food. Infected persons excrete high amounts of Astrovirus particles with their faeces (1, 2).The detection of Astrovirus may be performed by electron microscopy or by molecular biology techniques such as polymerase chain reaction (PCR). Meanwhile immunological methods like enzyme immunoassay have established as preferential methods for routine laboratory methods since these are fast, safe and automation is possible (1).
Principle of the Assay: The Astrovirus is a one-step enzyme immunoassay on the basis of polyclonal and monoclonal antibodies against Astrovirus antigens. Diluted stool specimens and horseradish peroxidase (HRP) labelled monoclonal anti-Astrovirus-antibodies are dispensed simultaneously into the wells of a microtitration plate coated with polyclonal anti-Astrovirus-antibodies. After an incubation time of 60 min at room temperature (RT) unbound components are removed by a washing step. HRP converts the subsequently added colourless substrate solution of 3,3',5,5'-Tetramethylbenzidine (TMB) within a 10 min reaction time at room temperature protected from light into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 / >= 620 nm is directly proportional to the specifically bound amount of Astrovirus!!Background/Introduction: Astrovirus was firstly described in 1975 and named according to its star-shaped structure visible under the electron microscope. Astrovirus belongs to the family Astroviridae. Human Astroviruses are subdivided into 7 serotypes (1). Together with Rotavirus and Adenovirus Astrovirus is one of the most common causes of non-bacterial gastroenteritis in children under 5 years of age all over the world. Thus 80% of children between 5 and 10 years of age are anti-Astrovirus-antibody positive. Astrovirus caused gastroenteritis in adults and nosocomial infections are observed as well (2). The course of the disease is usually self-limiting and of short duration. After the incubation time of 1-2 days a 1-4 days lasting gastroenteritis develops accompanied by vomiting, diarrhea, fever and abdominal pain finally causing dehydration. Although occurring all over the year Astrovirus infections are mainly observed during the winter months (3, 4). Astrovirus infections are spread via faecal-oral transmission from person to person or via contaminated things or food. Infected persons excrete high amounts of Astrovirus particles with their faeces (1, 2).The detection of Astrovirus may be performed by electron microscopy or by molecular biology techniques such as polymerase chain reaction (PCR). Meanwhile immunological methods like enzyme immunoassay have established as preferential methods for routine laboratory methods since these are fast, safe and automation is possible (1).
Product Categories/Family for BAFF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
29,826 Da
NCBI Official Full Name
BAFF
UniProt Protein Name
BAFF
UniProt Entry Name
A0A067XGS5_CARAU
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Product Notes
The BAFF (Catalog #AAA495312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BAFF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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