Typical Testing Data/Standard Curve (for reference only)
Rat cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit | CDH1 elisa kit
Rat Epithelial-Cadherin, E-Cad ELISA Kit
Reactivity
Rat
Synonyms
cadherin 1; type 1; E-cadherin (epithelial); Rat Epithelial-Cadherin; E-Cad ELISA Kit; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; E-Cadherin; E-cadherin (epithelial) ; calcium-dependent adhesion protein; epithelial; cell-CAM 120/80; uvomorulin; CDH1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat CDH1. No significant cross-reactivity or interference between Rat CDH1 and analogues was observed.
Sequence Length
886
Samples
Serum, plasma, tissue homogenates, Cell lysates
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
0.78 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Adhesion
Protein Biological Process 3
Cell adhesion
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CDH1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-Cad. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E-Cad and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E-Cad in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,715 Da
NCBI Official Full Name
cadherin-1
NCBI Official Synonym Full Names
cadherin 1
NCBI Official Symbol
Cdh1
NCBI Protein Information
cadherin-1; E-cadherin; uvomorulin; epithelial cadherin
UniProt Protein Name
Cadherin-1
Protein Family
UniProt Gene Name
Cdh1
UniProt Synonym Gene Names
E-cadherin
UniProt Entry Name
CADH1_RAT
NCBI Description
cell-cell adhesion molecule; may play a role in axonal growth and synase formation [RGD, Feb 2006]
Uniprot Description
Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7
Research Articles on CDH1
Similar Products
Product Notes
The Rat CDH1 cdh1 (Catalog #AAA703348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703348 ELISA Kit recognizes Rat CDH1. It is sometimes possible for the material contained within the vial of "cadherin 1, type 1, E-cadherin (epithelial), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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