Mouse E-Cadherin ELISA Kit | CDH1 elisa kit

Mouse E-Cadherin PicoKine ELISA Kit

Cat. Num. AAA175920

• Gene Names: Cdh1; Um; UVO; Ecad; E-cad; L-CAM; AA960649
• Reactivity: Mouse
• Synonyms: E-Cadherin; Mouse E-Cadherin PicoKine ELISA Kit; Cadherin-1; cadherin 1; type 1; E-cadherin (epithelial); Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; CDH1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse E-Cadherin
• Sequence Length: 884

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse E-Cadherin PicoKine ELISA Kit standard curve )

Related Product Information for CDH1 elisa kit

Principle of the assay: mouse E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for E-Cadherin has been precoated onto 96-well plates. Standards(NSO, D157-V709) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse E-Cadherin amount of sample captured in plate.
Background: E-Cadherin, also called Cadherin 1(CDH1),Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.

NCBI and Uniprot Product Information

• NCBI GI #: 115419
• NCBI GeneID: 12550
• UniProt Accession #: P09803; Q61377
• Molecular Weight: 98,256 Da
• NCBI Official Full Name: Cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Official Synonym Symbols: Um; UVO; Ecad; E-cad; L-CAM; AA960649
• NCBI Protein Information: cadherin-1; ARC-1; E-cadherin; uvomorulin; epithelial cadherin
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_MOUSE

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Ubiquitin ligase; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Cellular Component: apical junction complex; Golgi apparatus; internal side of plasma membrane; focal adhesion; cell surface; basolateral plasma membrane; lateral loop; integral to membrane; catenin complex; trans-Golgi network; intercellular junction; actin cytoskeleton; cell-cell adherens junction; adherens junction; membrane; axon; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nerve terminal; cell junction; lateral plasma membrane; endosome

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; metal ion binding; beta-catenin binding; GTPase activating protein binding; calcium ion binding; cell adhesion molecule binding; ankyrin binding; glycoprotein binding; protein phosphatase binding

Biological Process: in utero embryonic development; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of epithelial cell proliferation; trophectodermal cell differentiation; regulation of water loss via skin; cell-cell adhesion; sensory perception of sound; regulation of protein localization; synaptogenesis; calcium-dependent cell-cell adhesion; protein metabolic process; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; homophilic cell adhesion; cell adhesion; protein homooligomerization; negative regulation of epithelial cell proliferation