Rat E Cadherin ELISA Kit | CDH1 elisa kit

Rat E Cadherin ELISA Kit

Cat. Num. AAA8248473

• Reactivity: Rat
• Synonyms: E Cadherin; Rat E Cadherin ELISA Kit; CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDH1 elisa kit

• Reactivity: Rat
• Specificity: The Rat E Cadherin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat E Cadherin proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 886

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDH1 elisa kit

Principle of the Assay: The Rat E Cadherin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat E Cadherin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat E Cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and E Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat E Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 13786164
• NCBI GeneID: 83502
• NCBI Accession #: NP_112624.1
• NCBI GenBank Nucleotide #: NM_031334.1
• UniProt Accession #: Q9R0T4; O35794; Q9JIV9
• Molecular Weight: 98,715 Da
• NCBI Official Full Name: cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_RAT

NCBI Description

cell-cell adhesion molecule; may play a role in axonal growth and synase formation [RGD, Feb 2006]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Ubiquitin ligase

Cellular Component: actin cytoskeleton; adherens junction; apical junction complex; apical part of cell; axon; basolateral plasma membrane; catenin complex; cell junction; cell surface; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasm; flotillin complex; focal adhesion; integral to membrane; intercellular junction; internal side of plasma membrane; lamellipodium; lateral loop; lateral plasma membrane; nerve terminal; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: alpha-catenin binding; ankyrin binding; beta-catenin binding; calcium ion binding; cell adhesion molecule binding; cytoskeletal protein binding; gamma-catenin binding; glycoprotein binding; GTPase activating protein binding; protein binding; protein domain specific binding; protein phosphatase binding

Biological Process: calcium-dependent cell-cell adhesion; cell-cell adhesion; decidualization; embryo implantation; in utero embryonic development; negative regulation of cell-cell adhesion; negative regulation of epithelial cell proliferation; neurite development; pituitary gland development; positive regulation of cell-cell adhesion; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-dependent; protein homooligomerization; protein metabolic process; regulation of caspase activity; regulation of protein localization; regulation of water loss via skin; response to drug; response to organic substance; response to toxin; sensory perception of sound; synaptogenesis; trophectodermal cell differentiation