Rat CD80/B7-1 ELISA Kit | CD80 elisa kit

Rat CD80/B7-1 PicoKine ELISA Kit

Cat. Num. AAA177137

• Gene Names: Cd80; B7-1
• Reactivity: Rat
• Synonyms: CD80/B7-1; Rat CD80/B7-1 PicoKine ELISA Kit; T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; CD80 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat CD80
• Sequence Length: 321

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat CD80/B7-1 PicoKine ELISA Kit standard curve )

Related Product Information for CD80 elisa kit

Principle of the assay: rat CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, I39-Q248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD80 amount of sample captured in plate.
Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

NCBI and Uniprot Product Information

• NCBI GI #: 81908343
• NCBI GeneID: 25408
• UniProt Accession #: O35187
• Molecular Weight: 36,352 Da
• NCBI Official Full Name: B7.1
• NCBI Official Synonym Full Names: Cd80 molecule
• NCBI Official Symbol: Cd80
• NCBI Official Synonym Symbols: B7-1
• NCBI Protein Information: T-lymphocyte activation antigen CD80; CD80 antigen; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1)
• UniProt Protein Name: B7.1
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd80
• UniProt Entry Name: O35187_RAT

NCBI Description

cell surface protein that is part of the immune response; may be involved in induction of experimental autoimmune anterior uveitis [RGD, Feb 2006]