Typical Testing Data/Standard Curve (for reference only)
Human Antithrombin III ELISA Kit | SERPINC1 elisa kit
Human Antithrombin III ELISA Kit
Gene Names
SERPINC1; AT3; AT3D; ATIII; THPH7
Reactivity
Human
Synonyms
Antithrombin III; Human Antithrombin III ELISA Kit; AT3; Antithrombin-III; ATIII; Serpin C1; SERPINC1 elisa kit
Reactivity
Human
Specificity
The Human Antithrombin III ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Antithrombin III proteins within the range of 312 pg/ml - 20000 pg/ml.
Sequence Length
464
Samples
Cell Culture Supernates, Serum, Plasma, Urine
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINC1 elisa kit
Principle of the Assay: The Human Antithrombin III ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Antithrombin III in Cell Culture Supernates, Serum, Plasma, Urine. This assay employs an antibody specific for Human Antithrombin III coated on a 96-well plate. Standards and samples are pipetted into the wells and Antithrombin III present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Antithrombin III antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Antithrombin III bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).
Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,602 Da
NCBI Official Full Name
antithrombin-III
NCBI Official Synonym Full Names
serpin family C member 1
NCBI Official Symbol
SERPINC1
NCBI Official Synonym Symbols
AT3; AT3D; ATIII; THPH7
NCBI Protein Information
antithrombin-III
UniProt Protein Name
Antithrombin-III
Protein Family
UniProt Gene Name
SERPINC1
UniProt Synonym Gene Names
AT3; ATIII
UniProt Entry Name
ANT3_HUMAN
NCBI Description
The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]
Uniprot Description
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Research Articles on SERPINC1
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Product Notes
The Human SERPINC1 serpinc1 (Catalog #AAA8248398) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248398 ELISA Kit recognizes Human SERPINC1. It is sometimes possible for the material contained within the vial of "Antithrombin III, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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