Western Blot (WB)
(Anti- CD80 antibody, MBS176205, Western blottingAll lanes: Anti CD80 (MBS176205) at 0.5ug/mlWB: Rat Thymus Tissue Lysate at 50ugPredicted bind size: 34KDObserved bind size: 50KD )
Rabbit anti-Rat CD80 Polyclonal Antibody | anti-CD80 antibody
Anti-CD80 antibody
Reactivity
Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
CD80; Polyclonal Antibody; Anti-CD80 antibody; T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7 1 antigen antibody; Activation B7-1 antigen antibody; Activation B71 antigen antibody; B lymphocyte activation antigen B7 antibody; B7 antibody; B7-1 antibody; B7-1 antigen antibody; BB1 antibody; CD 80 antibody; CD28 antigen ligand 1 antibody; CD28 antigen ligand 1 B7 1 antigen antibody; CD28 antigen ligand 1 B71 antigen antibody; CD28LG antibody; CD28LG1 antibody; CD80 antibody; CD80 antigen antibody; CD80 antigen precursor antibody; CD80 molecule antibody; CD80_HUMAN antibody; Costimulatory factor CD80 antibody; CTLA 4 counter receptor B7.1 antibody; CTLA-4 counter-receptor B7.1 antibody; LAB 7 antibody; LAB7 antibody; T lymphocyte activation antigen CD80 antibody; T-lymphocyte activation antigen CD80 antibody; anti-CD80 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
321
Applicable Applications for anti-CD80 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80 (227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- CD80 antibody, MBS176205, Western blottingAll lanes: Anti CD80 (MBS176205) at 0.5ug/mlWB: Rat Thymus Tissue Lysate at 50ugPredicted bind size: 34KDObserved bind size: 50KD )
Western Blot (WB)
(Anti- CD80 antibody, MBS176205, Western blottingAll lanes: Anti CD80 (MBS176205) at 0.5ug/mlWB: Rat Thymus Tissue Lysate at 50ugPredicted bind size: 34KDObserved bind size: 50KD )
Related Product Information for anti-CD80 antibody
Description: Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.
Background: Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Background: Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
36,298 Da
NCBI Official Full Name
CD80
UniProt Protein Name
CD80
Protein Family
UniProt Entry Name
O55202_RAT
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Product Notes
The CD80 (Catalog #AAA176205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD80 antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD80 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CD80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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