Human T-lymphocyte activation antigen CD80 ELISA Kit | CD80 elisa kit

Human T-lymphocyte activation antigen CD80 ELISA Kit

Cat. Num. AAA760940

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Reactivity: Human
• Synonyms: T-lymphocyte activation antigen CD80; Human T-lymphocyte activation antigen CD80 ELISA Kit; CD80/B7-1/T-lymphocyte activation antigen CD80; CD80 elisa kit

• Reactivity: Human
• Sequence Length: 288

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CD80 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CD80 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CD80 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD80 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CD80 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182.1
• NCBI GenBank Nucleotide #: NM_005191.3
• Molecular Weight: 18,761 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7
• UniProt Entry Name: CD80_HUMAN

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: cell surface; external side of plasma membrane; integral to membrane; intracellular; plasma membrane

Molecular Function: coreceptor activity; protein binding; viral receptor activity

Biological Process: cell-cell signaling; entry of virus into host cell; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of alpha-beta T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-2 biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of signal transduction; positive regulation of T-helper 1 cell differentiation; positive regulation of transcription, DNA-dependent; T cell activation; T cell costimulation