Typical Testing Data/Standard Curve (for reference only)
Rat Bromodomain-containing protein 4 (BRD4) ELISA Kit | BRD4 elisa kit
Rat Bromodomain-containing protein 4 (BRD4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BRD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BRD4 antibody and an BRD4-HRP conjugate. The assay sample and buffer are incubated together with BRD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BRD4 concentration since BRD4 from samples and BRD4-HRP conjugate compete for the anti-BRD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by BRD4 from the sample, fewer sites are left to bind BRD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BRD4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family
Chromosomal Location of Human Ortholog: 19p13.1
Cellular Component: nucleoplasm; transcription elongation factor complex b; condensed nuclear chromosome; cytoplasm; nuclear chromatin; chromosome; nucleus
Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; viral reproduction; protein amino acid phosphorylation; inner cell mass cell proliferation; chromosome segregation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding
Research Articles on BRD4
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Product Notes
The Rat BRD4 brd4 (Catalog #AAA7218501) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218501 ELISA Kit recognizes Rat BRD4. It is sometimes possible for the material contained within the vial of "Bromodomain-containing protein 4 (BRD4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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