Goat anti-Human Monoglyceride Lipase Polyclonal Antibody | anti-MGLL antibody

Monoglyceride Lipase, ID (MGLL, MGL, HU-K5, Lysophospholipase Homolog, Lysophospholipase-like, Monoacylglycerol Lipase, MAGL)

Cat. Num. AAA6003781

• Gene Names: MGLL; MGL; HUK5; MAGL; HU-K5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Monoglyceride Lipase; Polyclonal Antibody; ID (MGLL; MGL; HU-K5; Lysophospholipase Homolog; Lysophospholipase-like; Monoacylglycerol Lipase; MAGL); Anti -Monoglyceride Lipase; anti-MGLL antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human MGLL. Species sequence homology: bovine, canine, mouse, porcine and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-MGLL antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 0.5-1.5ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide C-QDLPHLVNADGQY from an internal region (near N-terminus) of human MGLL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(Antibody (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-MGLL antibody

MAG Lipase is a lipase that functions together with hormone-sensitive lipase to hydrolyze intracellular triglyceride stores in adipocytes and other cell types to form fatty acids and glycerol. MAG lipase also works with lipoprotein lipase to hydrolyze monoglycerides resulting from degradation of lipoprotein triglycerides. It is localized in the enteric nervous system to function in the regulation of levels of endocannabinoids, and regulates intestinal motility and has a role in digestion.

Product Categories/Family for anti-MGLL antibody

Antibodies; Abs to Enzymes; Lipase

NCBI and Uniprot Product Information

• NCBI GI #: 14594904
• NCBI GeneID: 11343
• UniProt Accession #: Q99685; Q6IBG9; Q96AA5; B3KRC2; B7Z9D1
• Molecular Weight: 33,261 Da
• NCBI Official Full Name: monoglyceride lipase
• NCBI Official Synonym Full Names: monoglyceride lipase
• NCBI Official Symbol: MGLL
• NCBI Official Synonym Symbols: MGL; HUK5; MAGL; HU-K5
• NCBI Protein Information: monoglyceride lipase; monoacylglycerol lipase; lysophospholipase homolog
• UniProt Protein Name: Monoglyceride lipase
• Protein Family: Monoglyceride lipase
• UniProt Gene Name: MGLL
• UniProt Synonym Gene Names: MGL; MAGL
• UniProt Entry Name: MGLL_HUMAN

NCBI Description

This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

Protein type: Phospholipase; Lipid Metabolism - glycerolipid; EC 3.1.1.23

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol

Molecular Function: protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity

Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; triacylglycerol catabolic process; regulation of inflammatory response; arachidonic acid metabolic process; lipid metabolic process; regulation of sensory perception of pain; blood coagulation; inflammatory response; fatty acid biosynthetic process; acylglycerol catabolic process