Mouse anti-Mouse, Human hnRNP A1 Monoclonal Antibody | anti-hnRNP A1 antibody

Mouse Anti-hnRNP A1 [9H10]

Cat. Num. AAA375248

• Reactivity: Mouse, Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: hnRNP A1; Monoclonal Antibody; Mouse Anti-hnRNP A1 [9H10]; HNRNPA 1 antibody; Helix destabilizing protein antibody; Helix-destabilizing proteinantibody; Heterogeneous nuclear ribonucleoprotein A1 antibody; Heterogeneous nuclear ribonucleoprotein A1B proteinantibody; Heterogeneous nuclear ribonucleoprotein B2 protein antibody; Heterogeneous nuclear ribonucleoprotein coreprotein A1 antibody; hnRNP A1 antibody; hnRNP core protein A1 antibody; HNRNPA1 antibody; HNRPA1antibody; MGC102835 antibody; Nuclear ribonucleoprotein particle A1 protein antibody; ROA1_HUMANantibody; anti-hnRNP A1 antibody

• Host: Mouse
• Reactivity: Mouse, Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9H10
• Purity/Purification: Protein A
• Form/Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2

• Applicable Applications for anti-hnRNP A1 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try; Flow Cyt: Use 1ug for 106 cells. IHC-P: Use a concentration of 1 ug/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol WB: Use at 1:2000-1:4000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa) IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex
• Target Antigen: hnRNP A1
• Immunogen: Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human)
• Myeloma/fusion Partners: Balb/c Sp2/0 cell line
• Research Areas: RNA binding; RNA Processing

Immunohistochemistry (IHC)

References

RNA binding specificity of hnRNP A1: significance of hnRNP A1 highaffinity binding sites in premRNA splicing. Burd CG (Embo J,Mar 1994) PUBMED Kashima T et al. hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing. Hum Mol Genet. 2007 Dec 15;16(24):3149-59. Epub 2007 Sep 19. WB, IP; HumanPMID: 17884807 Woolley AG et al. Prognostic Association of YB-1 Expression in Breast Cancers: A Matter of Antibody. PLoS One 6:e20603 (2011). ICC/IF ; Human PUBMED Kocgozlu L et al. Selective and uncoupled role of substrate elasticity in the regulation of replication and transcription in epithelial cells. J Cell Sci 123:29-39 (2010). ICC/IF ; Marsupials PUBMED Rosenberger S et al. Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation. Proc Natl Acad Sci U S A : (2010). WB ; Human PUBMED Sellier C et al. Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. EMBO J 29:1248-61 (2010) PUBMED Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010). WB, In Situ Hybridisation ; Human PUBMED Beauchamp P et al. The cleavage of HuR interferes with its transportin-2-mediated nuclear import and promotes muscle fiber formation. Cell Death Differ 17:1588-99 (2010). WB ; Mouse PUBMED Lieberthal JG et al. The role of YY1 in reduced HP1alpha gene expression in invasive human breast cancer cells. Breast Cancer Res 11:R42 (2009). WB ; Human PUBMED van der Giessen K & Gallouzi IE Involvement of transportin 2-mediated HuR import in muscle cell differentiation. Mol Biol Cell 18:2619-29 (2007). WB, ICC/IF ; Mouse PUBMED

NCBI and Uniprot Product Information

• NCBI GI #: 4433158
• NCBI Official Full Name: hnRNP A1, partial