SDS-Page
BRD4 recombinant protein
Recombinant Human BRD4 (N-10His-DYKDDDDK)
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Purity
>95% as determined by reducing SDS-PAGE
Synonyms
BRD4; Recombinant Human BRD4 (N-10His-DYKDDDDK); HUNK1; HUNK1bromodomain-containing protein 4; HUNKI; MCAP; BRD4 recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by reducing SDS-PAGE
Form/Format
Supplied as a 0.2um filtered solution of 50mM HEPES, 200mM NaCl, 1mM DTT, 10% Glycerol, pH7.5.
Sequence
Glu49-Glu460
Species
Human
Tag
N-10His-DYKDDDDK
Endotoxin
<1.0EU per ug as determined by the LAL method.
Protein Construction
Recombinant Human Bromodomain-containing protein 4 is produced by our E Coli expression system and the target gene encoding Glu49-Glu460 is expressed with a 10His, DYKDDDDK tag at the N-terminus.
Preparation and Storage
Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20 degree C.
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20 degree C.
SDS-Page
SDS-Page
Related Product Information for BRD4 recombinant protein
Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
Product Categories/Family for BRD4 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
152kDa
NCBI Official Full Name
bromodomain-containing protein 4 isoform short
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
BRD4
NCBI Official Synonym Symbols
CAP; MCAP; HUNK1; HUNKI
NCBI Protein Information
bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
UniProt Protein Name
Bromodomain-containing protein 4
Protein Family
UniProt Gene Name
BRD4
UniProt Synonym Gene Names
HUNK1
UniProt Entry Name
BRD4_HUMAN
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Product Notes
The BRD4 brd4 (Catalog #AAA2569165) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Glu49-Glu4 60. It is sometimes possible for the material contained within the vial of "BRD4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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