Typical Testing Data/Standard Curve (for reference only)
Rat Alpha-1-Antitrypsin (a1AT) ELISA Kit | a1AT elisa kit
Rat Alpha-1-Antitrypsin (a1AT)(Sandwich ELISA) ELISA Kit
This assay has high sensitivity and excellent specificity for detection of Mini Samples Alpha-1-Antitrypsin (a1AT). No significant cross-reactivity or interference between Mini Samples Alpha-1-Antitrypsin (a1AT) and analogues was observed.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
Uniprot Description
SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Inhibitor; Secreted, signal peptide
Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus
Molecular Function: endopeptidase inhibitor activity; glycoprotein binding; identical protein binding; protease binding; protein binding; serine-type endopeptidase inhibitor activity
Biological Process: acute-phase response; inflammatory response; response to chromate; response to cytokine stimulus; response to estradiol stimulus; response to hypoxia; response to inorganic substance; response to lead ion; response to lipopolysaccharide; response to methanol; response to organic cyclic substance; response to peptide hormone stimulus; response to triglyceride
Research Articles on a1AT
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Product Notes
The Rat a1AT serpina1 (Catalog #AAA2709658) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709658 ELISA Kit recognizes Rat a1AT. AAA Biotech's Alpha-1-Antitrypsin (a1AT) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the a1AT serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-1-Antitrypsin (a1AT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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