Human Asporin (ASPN) ELISA Kit | ASPN elisa kit

Human Asporin (ASPN) ELISA Kit

Cat. Num. AAA2704362

• Gene Names: ASPN; OS3; PLAP1; PLAP-1; SLRR1C
• Reactivity: Human
• Synonyms: Asporin (ASPN); Human Asporin (ASPN) ELISA Kit; PLAP1; SLRR1C; Periodontal ligament-associated protein 1; ASPN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of asporin. No significant cross-reactivity or interference between asporin and analogues was observed.
• Sequence Length: 380

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level asporin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level asporin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASPN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of asporin in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to asporin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to asporin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain asporin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of asporin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13625797
• NCBI GeneID: 54829
• UniProt Accession #: Q9BXN1; Q5TBF3; Q96K79; Q96LD0; Q9NXP3
• Molecular Weight: 43,417 Da
• NCBI Official Full Name: asporin
• NCBI Official Synonym Full Names: asporin
• NCBI Official Symbol: ASPN
• NCBI Official Synonym Symbols: OS3; PLAP1; PLAP-1; SLRR1C
• NCBI Protein Information: asporin
• UniProt Protein Name: Asporin
• Protein Family: Asporin
• UniProt Gene Name: ASPN
• UniProt Synonym Gene Names: PLAP1; SLRR1C; PLAP-1

NCBI Description

This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis, and also with intervertebral disc disease. Alternative splicing of this gene results in multiple transcript variants.[provided by RefSeq, Jul 2014]

Uniprot Description

Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.