Rat A1AT (alpha 1-Antitrypsin) ELISA Kit | A1AT elisa kit

Rat A1AT (alpha 1-Antitrypsin) ELISA Kit

Cat. Num. AAA7608253

• Gene Names: Serpina1; Pi; AAT; Spi1
• Reactivity: Rat
• Synonyms: A1AT (alpha 1-Antitrypsin); Rat A1AT (alpha 1-Antitrypsin) ELISA Kit; alpha1-AT; A1AT; alpha 1-Antitrypsin; Serpin A1; alpha 1-Proteinase Inhibitor; A1A; AATMGC23330; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha-1-antitrypsin; antitrypsin; A1AT elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of A1AT. No significant cross-reactivity or interference between A1AT and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000ng/ml
• Sensitivity: 18.75ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for A1AT elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 51036655
• NCBI GeneID: 24648
• NCBI Accession #: NP_071964.2
• NCBI GenBank Nucleotide #: NM_022519.2
• UniProt Accession #: P17475; Q6AYZ5
• Molecular Weight: 46,136 Da
• NCBI Official Full Name: alpha-1-antiproteinase
• NCBI Official Synonym Full Names: serpin family A member 1
• NCBI Official Symbol: Serpina1
• NCBI Official Synonym Symbols: Pi; AAT; Spi1
• NCBI Protein Information: alpha-1-antiproteinase
• UniProt Protein Name: Alpha-1-antiproteinase
• UniProt Gene Name: Serpina1
• UniProt Entry Name: A1AT_RAT

Uniprot Description

SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus

Molecular Function: endopeptidase inhibitor activity; glycoprotein binding; identical protein binding; protease binding; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; inflammatory response; response to chromate; response to cytokine stimulus; response to estradiol stimulus; response to hypoxia; response to inorganic substance; response to lead ion; response to lipopolysaccharide; response to methanol; response to organic cyclic substance; response to peptide hormone stimulus; response to triglyceride